{"EMPIAR-10363":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe data for 2.3 Angstrom cryo-EM reconstructions of HemQ from Geobacillus in the presence of substantial aberrations","directory":"data/T0T_grid6","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":258,"frames_per_image":100,"frame_range_min":1,"frame_range_max":100,"voxel_type":"UNSIGNED BYTE","pixel_width":0.36,"pixel_height":0.36,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Image height and width may have to be swapped.","image_width":"7676","image_height":"7420"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"2.3 Angstrom cryo-EM reconstructions of HemQ from Geobacillus","principal_investigator":[{"author_orcid":"0000-0002-4321-6253","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","telephone":"2146459577","fax":null,"first_name":"Dominika","last_name":"Borek","email":"dominika [at] work.swmed.edu","country":"United States","entry":"EMPIAR-10363"},{"author_orcid":"0000-0003-3640-8545","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","telephone":"2146456385","fax":null,"first_name":"Zbyszek","last_name":"Otwinowski","email":"zbyszek [at] work.swmed.edu","country":"United States","entry":"EMPIAR-10363"}],"status":"REL","deposition_date":"2020-02-13","release_date":"2020-05-26","obsolete_date":null,"update_date":"2021-09-07","corresponding_author":{"author":{"author_orcid":"0000-0002-4321-6253","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","first_name":"Dominika","last_name":"Borek","country":"United States"}},"authors":[{"author":{"name":"Bromberg R","author_orcid":"0000-0003-3526-6362"}},{"author":{"name":"Guo Y","author_orcid":"0000-0001-7705-5454"}},{"author":{"name":"Borek D","author_orcid":"0000-0002-4321-6253"}},{"author":{"name":"Otwinowski Z","author_orcid":"0000-0003-3640-8545"}}],"cross_references":["EMD-21373"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Bromberg R","author_orcid":"0000-0003-3526-6362"},{"name":"Guo Y","author_orcid":"0000-0001-7705-5454"},{"name":"Borek D","author_orcid":"0000-0002-4321-6253"},{"name":"Otwinowski Z","author_orcid":"0000-0003-3640-8545"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"High-resolution cryo-EM reconstructions in the presence of substantial aberrations","volume":"7","country":"","first_page":"445","last_page":"452","year":"2020","language":"English","doi":"10.1107/s2052252520002444","pubmedid":null,"details":"1. Talos Arctica 200 kV, K2 Gatan, detector pixel size 0.72 Angstrom (nominal mag 57k), data pixel size 0.36, no phase plate, no objective aperture (due to instability of its position during data collection), \n2. large defocus (-2.8 to -1.1 um) , large coma (6.9 mrad), trefoil (0.49 um), anisotropic magnification (~3%)\n3. 258 unaligned movies, each contains 100 raw movie frames with frames exposed 0.4 s/frame with dose ~0.9 electron per squared Angstrom per frame\n5. Particle symmetry - C5, mass 144 kDa, very strong preferred orientation\n6. Final reconstruction without polishing 2.32 Angstrom (0.143 FSC criterion)","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"IUCrJ","journal_abbreviation":"IUCrJ","issue":"Pt 3","preprint":false}],"dataset_size":"1.4 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6vsa"],"entry_doi":"10.6019/EMPIAR-10363"}}