{"EMPIAR-10360":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe data for xylose isomerase (glucose isomerase)","directory":"data","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":202,"frames_per_image":200,"frame_range_min":1,"frame_range_max":200,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.91,"pixel_height":0.91,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Image width and height may need to be swapped.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"2.7 Angstrom cryo-EM reconstructions of glucose isomerase in the presence of substantial aberrations","principal_investigator":[{"author_orcid":"0000-0003-3640-8545","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","telephone":"2146456385","fax":null,"first_name":"Zbyszek","last_name":"Otwinowski","email":"zbyszek [at] work.swmed.edu","country":"United States","entry":"EMPIAR-10360"},{"author_orcid":"0000-0002-4321-6253","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","telephone":"2146459577","fax":null,"first_name":"Dominika","last_name":"Borek","email":"dominika [at] work.swmed.edu","country":"United States","entry":"EMPIAR-10360"}],"status":"REL","deposition_date":"2020-02-10","release_date":"2020-05-27","obsolete_date":null,"update_date":"2020-05-27","corresponding_author":{"author":{"author_orcid":"0000-0002-4321-6253","middle_name":null,"organization":"UT Southwestern Medical Center","street":"5323 Harry Hines Blvd.","town_or_city":"Dallas","state_or_province":"TX","post_or_zip":"75390","first_name":"Dominika","last_name":"Borek","country":"United States"}},"authors":[{"author":{"name":"Bromberg R","author_orcid":"0000-0003-3526-6362"}},{"author":{"name":"Guo Y","author_orcid":"0000-0001-7705-5454"}},{"author":{"name":"Borek D","author_orcid":"0000-0002-4321-6253"}},{"author":{"name":"Otwinowski Z","author_orcid":"0000-0003-3640-8545"}}],"cross_references":["EMD-21371"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Bromberg R","author_orcid":"0000-0003-3526-6362"},{"name":"Guo Y","author_orcid":"0000-0001-7705-5454"},{"name":"Borek D","author_orcid":"0000-0002-4321-6253"},{"name":"Otwinowski Z","author_orcid":"0000-0003-3640-8545"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"High-resolution cryo-EM reconstructions in the presence of substantial aberrations","volume":"7","country":"","first_page":"445","last_page":"452","year":"2020","language":"English","doi":"10.1107/s2052252520002444","pubmedid":null,"details":"1. Talos Arctica 200 kV, K2 Gatan, detector pixel size 0.91 Angstrom (nominal mag 45k),  no phase plate, no objective aperture (due to instability of its position during data collection), \n2. large defocus () , large coma (5.2 mrad), minimal trefoil (0.49 um), anisotropic magnification ()\n3. 202 unaligned movies, each contains 200 raw movie frames with frames exposed 0.5 s/frame with dose ~0.6 electron per squared Angstrom per frame\n5. Particle symmetry - D2, mass 172 kDa\n6. Final reconstruction without polishing 2.7 Angstrom (0.143 FSC criterion)","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"IUCrJ","journal_abbreviation":"IUCrJ","issue":"Pt 3","preprint":false}],"dataset_size":"1.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6vrs"],"entry_doi":"10.6019/EMPIAR-10360"}}