{"EMPIAR-10358":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5172,"frames_per_image":48,"frame_range_min":1,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":1.15,"pixel_height":1.15,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies were recorded automatically using EPU on a K2 camera in counting mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 10.8 second movies contain 48 frames and the pixel size is 1.15 Å/px. The total dose per movie is ~47.5 e/Å^2. The gain reference image is included.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Dose-weighted aligned micrographs","directory":"data/Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5172,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.15,"pixel_height":1.15,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movie frames were corrected for beam-induced motion and dose-weighted using RELION’s motion-correction implementation with following arguments:\n--first_frame_sum 1 --last_frame_sum -1 --use_own  --j 5 --bin_factor 1 --bfactor 150 --angpix 1.15 --voltage 300 --dose_per_frame 0.81 --preexposure 0 --patch_x 4 --patch_y 4 --gainref gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Polished particle stacks","directory":"data/Particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":3030,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.15,"pixel_height":1.15,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Bayesian polishing was performed in RELION-3.0. STAR files of both Type2-1 and Type2-2 with the metadata of the final refined segments are included.","image_width":"220","image_height":"220"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 2","principal_investigator":[{"author_orcid":"0000-0002-5214-7886","middle_name":null,"organization":"MRC-LMB","street":"Francis Crick Avenue, Cambridge Biomedical Campus","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Michel","last_name":"Goedert","email":"mg [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10358"},{"author_orcid":"0000-0002-0462-6540","middle_name":"H.W.","organization":"MRC-LMB","street":"Francis Crick Avenue, Cambridge Biomedical Campus","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Sjors","last_name":"Scheres","email":"scheres [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10358"}],"status":"REL","deposition_date":"2020-02-07","release_date":"2020-11-18","obsolete_date":null,"update_date":"2020-12-09","corresponding_author":{"author":{"author_orcid":"0000-0003-1579-7561","middle_name":null,"organization":"MRC-LMB","street":"Francis Crick Avenue, Cambridge Biomedical Campus","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Yang","last_name":"Shi","country":"United Kingdom"}},"authors":[{"author":{"name":"Schweighauser M","author_orcid":"0000-0002-1848-1610"}},{"author":{"name":"Shi Y","author_orcid":"0000-0003-1579-7561"}},{"author":{"name":"Tarutani A","author_orcid":null}},{"author":{"name":"Kametani F","author_orcid":"0000-0001-9125-7001"}},{"author":{"name":"Murzin AG","author_orcid":null}},{"author":{"name":"Ghetti B","author_orcid":"0000-0002-1842-8019"}},{"author":{"name":"Matsubara T","author_orcid":"0000-0002-7559-8284"}},{"author":{"name":"Tomita T","author_orcid":"0000-0002-0075-5943"}},{"author":{"name":"Ando T","author_orcid":null}},{"author":{"name":"Hasegawa K","author_orcid":null}},{"author":{"name":"Murayama S","author_orcid":null}},{"author":{"name":"Yoshida M","author_orcid":null}},{"author":{"name":"Hasegawa M","author_orcid":null}},{"author":{"name":"Scheres SHW","author_orcid":"0000-0002-0462-6540"}},{"author":{"name":"Goedert M","author_orcid":"0000-0002-5214-7886"}}],"cross_references":["EMD-10651","EMD-10652"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Schweighauser M","author_orcid":"0000-0002-1848-1610"},{"name":"Shi Y","author_orcid":"0000-0003-1579-7561"},{"name":"Tarutani A","author_orcid":null},{"name":"Kametani F","author_orcid":"0000-0001-9125-7001"},{"name":"Murzin AG","author_orcid":null},{"name":"Ghetti B","author_orcid":"0000-0002-1842-8019"},{"name":"Matsubara T","author_orcid":"0000-0002-7559-8284"},{"name":"Tomita T","author_orcid":"0000-0002-0075-5943"},{"name":"Ando T","author_orcid":null},{"name":"Hasegawa K","author_orcid":null},{"name":"Murayama S","author_orcid":null},{"name":"Yoshida M","author_orcid":null},{"name":"Hasegawa M","author_orcid":null},{"name":"Scheres SHW","author_orcid":"0000-0002-0462-6540"},{"name":"Goedert M","author_orcid":"0000-0002-5214-7886"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures of α-synuclein filaments from multiple system atrophy","volume":"585","country":"United Kingdom","first_page":"464","last_page":"469","year":"2020","language":"English","doi":"10.1038/s41586-020-2317-6","pubmedid":"32461689","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7825","preprint":false}],"dataset_size":"1.1 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6xyp","6xyq"],"entry_doi":"10.6019/EMPIAR-10358"}}