{"EMPIAR-10333":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs","directory":"data/01_Raw_Frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8319,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.0961,"pixel_height":1.0961,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned movies saved in TIF format. The first data collection (19feb16b) was performed using a dose of ~66.37 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~8.0 e–/pix/sec, using a set defocus range of -1.9 μm to -2.0 μm. The second data collection (19may31e) was performed using a dose of ~ 63.61 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~7.6 e–/pix/sec, using a set defocus range of -1.5 μm to -2.5 μm. The gain reference images for each data collection are in the respective references folder.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs","directory":"data/02_Aligned_Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6934,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.0961,"pixel_height":1.0961,"micrographs_file_pattern":"data/02_Aligned_Micrographs/MotionCorr/job0*/Movies/*mrc","picked_particles_file_pattern":"data/03_Particle_Stacks/Class*.star","picked_particles_directory":"data/03_Particle_Stacks","details":"Aligned and dose-weighted micrographs. Motion correction was performed using Relion 3.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Final Particle Stacks for Class 1 and 2 with Euler Angles and Shifts","directory":"data/03_Particle_Stacks","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":2,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.403008,"pixel_height":1.403008,"micrographs_file_pattern":"data/02_Aligned_Micrographs/MotionCorr/job0*/Movies/*mrc","picked_particles_file_pattern":"data/03_Particle_Stacks/Class*.star","picked_particles_directory":"data/03_Particle_Stacks","details":"Final particle stacks for both class 1 and 2 maps with corresponding Relion .star file containing the refined Euler angles and shifts.","image_width":"200","image_height":"200"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes","principal_investigator":[{"author_orcid":"0000-0001-5136-5331","middle_name":null,"organization":"Department of Biochemistry, University of Colorado at Boulder","street":"596 UCB","town_or_city":"Boulder","state_or_province":"Colorado","post_or_zip":"80305","telephone":"+1 303 735 6689","fax":null,"first_name":"Karolin","last_name":"Luger","email":"karolin.luger [at] colorado.edu","country":"United States","entry":"EMPIAR-10333"}],"status":"REL","deposition_date":"2019-10-21","release_date":"2019-12-20","obsolete_date":null,"update_date":"2021-11-08","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","first_name":"Yong Zi","last_name":"Tan","country":"United States"}},"authors":[{"author":{"name":"Liu YL","author_orcid":"0000-0001-7187-9196"}},{"author":{"name":"Zhou KZ","author_orcid":"0000-0003-0258-1489"}},{"author":{"name":"Zhang NZ","author_orcid":"0000-0003-1324-7099"}},{"author":{"name":"Wei HW","author_orcid":"0000-0001-6777-1772"}},{"author":{"name":"Tan YZT","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Zhang ZZ","author_orcid":"0000-0001-5104-4220"}},{"author":{"name":"Carragher BC","author_orcid":"0000-0002-0624-5020"}},{"author":{"name":"Potter CSP","author_orcid":"0000-0003-2394-0831"}},{"author":{"name":"D'Arcy SD","author_orcid":"0000-0001-5055-988X"}},{"author":{"name":"Luger KL","author_orcid":"0000-0001-5136-5331"}}],"cross_references":["EMD-20840","EMD-20841"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Liu YL","author_orcid":"0000-0001-7187-9196"},{"name":"Zhou KZ","author_orcid":"0000-0003-0258-1489"},{"name":"Zhang NZ","author_orcid":"0000-0003-1324-7099"},{"name":"Wei HW","author_orcid":"0000-0001-6777-1772"},{"name":"Tan YZT","author_orcid":"0000-0001-6656-6320"},{"name":"Zhang ZZ","author_orcid":"0000-0001-5104-4220"},{"name":"Carragher BC","author_orcid":"0000-0002-0624-5020"},{"name":"Potter CSP","author_orcid":"0000-0003-2394-0831"},{"name":"D'Arcy SD","author_orcid":"0000-0001-5055-988X"},{"name":"Luger KL","author_orcid":"0000-0001-5136-5331"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"FACT caught in the act of manipulating the nucleosome","volume":null,"country":"United Kingdom","first_page":null,"last_page":null,"year":"2019","language":"English","doi":"10.1038/s41586-019-1820-0","pubmedid":"31775157","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"2.2 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6upk","6upl"],"entry_doi":"10.6019/EMPIAR-10333"}}