{"EMPIAR-10330":{"imagesets":[{"segmentations":[],"name":"Unaligned and compressed multi-frame micrographs","directory":"data/01_Raw_Frames","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":3383,"frames_per_image":80,"frame_range_min":1,"frame_range_max":80,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.5175,"pixel_height":0.5175,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collection was performed using a dose of ~91.56 e-/Å2 across 80 frames (75 msec per frame) at a dose rate of ~4.0 e–/pix/sec, using a set defocus range of -1.2 μm to -1.8 μm. Raw frames were compressed using pbzip2. Gain reference image to use is 18jun08i_08213559_01_3710x3838_norm_0_mod.mrc. Magnification anisotropy is present (Distortion angle of 172.7 degrees, major scale of 1.007 and minor scale of 0.993). The script to correct for magnification anisotropy (mag_distort_correct4.cs is attached, and it requires the mag_distortion_correct software available from the Grigorieff lab website.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned and magnification anisotropy corrected micrographs","directory":"data/02_Aligned_Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3297,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.5175,"pixel_height":0.5175,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs aligned using MotionCor2, magnification anisotropy corrected. Alignment was done initially using 3x3 patches (Patch3_micrographs). Micrographs that had estimated resolution at 0.5 confidence level of worse than 10 Å were removed, resulting in 3,297 micrographs. Patch2_micrographs were used later during refinement - hence only a subset of micrographs were aligned using 2x2 patches (Patch2_micrographs).","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Final Particle Stack with Refined Euler Angles and Shifts","directory":"data/03_Final_Refined_Particle_Stack","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":16905,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.035,"pixel_height":1.035,"micrographs_file_pattern":"data/02_Aligned_Micrographs/Patch*_micrographs/*mrc","picked_particles_file_pattern":"data/03_Final_Refined_Particle_Stack/FinalRefinement-OriginalParticles-PfCRT.star","picked_particles_directory":"data/03_Final_Refined_Particle_Stack","details":"Final particle stack, with Relion .star file containing the refined Euler angles and shifts.","image_width":"300","image_height":"300"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"3.2 Å Single-Particle Cryo-EM Reconstruction of 49 kDa Membrane-Bound PfCRT Complexed with Fab","principal_investigator":[{"author_orcid":"0000-0003-3293-2200","middle_name":null,"organization":"Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center","street":null,"town_or_city":"New York","state_or_province":"New York","post_or_zip":"NY 10032","telephone":null,"fax":null,"first_name":"Filippo","last_name":"Mancia","email":"fm123 [at] cumc.columbia.edu","country":"United States","entry":"EMPIAR-10330"},{"author_orcid":"0000-0001-6753-8938","middle_name":"Armand","organization":"Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center","street":null,"town_or_city":"New York","state_or_province":"New York","post_or_zip":"NY 10032","telephone":null,"fax":null,"first_name":"David","last_name":"Fidock","email":"df2260 [at] cumc.columbia.edu","country":"United States","entry":"EMPIAR-10330"},{"author_orcid":"0000-0002-8620-827X","middle_name":null,"organization":"Department of Psychiatry, Center for Molecular Recognition, Columbia University Irving Medical Center","street":null,"town_or_city":"New York","state_or_province":"New York","post_or_zip":"NY 10032","telephone":null,"fax":null,"first_name":"Matthias","last_name":"Quick","email":"mq2102 [at] cumc.columbia.edu","country":"United States","entry":"EMPIAR-10330"}],"status":"REL","deposition_date":"2019-10-06","release_date":"2019-12-19","obsolete_date":null,"update_date":"2020-01-29","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","first_name":"Yong Zi","last_name":"Tan","country":"United States"}},"authors":[{"author":{"name":"Kim JK","author_orcid":"0000-0003-2594-3251"}},{"author":{"name":"Tan YZT","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Wicht KJW","author_orcid":"0000-0001-6145-9956"}},{"author":{"name":"Erramilli SKE","author_orcid":"0000-0001-8694-7681"}},{"author":{"name":"Dhingra SKD","author_orcid":"0000-0002-3893-1615"}},{"author":{"name":"Okombo JO","author_orcid":null}},{"author":{"name":"Vendome JV","author_orcid":null}},{"author":{"name":"Hagenah LMH","author_orcid":null}},{"author":{"name":"Giacometti SIG","author_orcid":null}},{"author":{"name":"Warren ALW","author_orcid":null}},{"author":{"name":"Nosol KN","author_orcid":"0000-0003-2865-9125"}},{"author":{"name":"Roepe PDR","author_orcid":"0000-0002-7079-3793"}},{"author":{"name":"Potter CSP","author_orcid":"0000-0002-3287-121X"}},{"author":{"name":"Carragher BC","author_orcid":"0000-0002-0624-5020"}},{"author":{"name":"Kossiakoff AAK","author_orcid":"0000-0003-3174-9359"}},{"author":{"name":"Quick MQ","author_orcid":"0000-0002-8620-827X"}},{"author":{"name":"Fidock DAF","author_orcid":"0000-0001-6753-8938"}},{"author":{"name":"Mancia FM","author_orcid":"0000-0003-3293-2200"}}],"cross_references":["EMD-20806"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kim JK","author_orcid":"0000-0003-2594-3251"},{"name":"Tan YZT","author_orcid":"0000-0001-6656-6320"},{"name":"Wicht KJW","author_orcid":"0000-0001-6145-9956"},{"name":"Erramilli SKE","author_orcid":"0000-0001-8694-7681"},{"name":"Dhingra SKD","author_orcid":"0000-0002-3893-1615"},{"name":"Okombo JO","author_orcid":null},{"name":"Vendome JV","author_orcid":null},{"name":"Hagenah LMH","author_orcid":null},{"name":"Giacometti SIG","author_orcid":null},{"name":"Warren ALW","author_orcid":null},{"name":"Nosol KN","author_orcid":"0000-0003-2865-9125"},{"name":"Roepe PDR","author_orcid":"0000-0002-7079-3793"},{"name":"Potter CSP","author_orcid":"0000-0002-3287-121X"},{"name":"Carragher BC","author_orcid":"0000-0002-0624-5020"},{"name":"Kossiakoff AAK","author_orcid":"0000-0003-3174-9359"},{"name":"Quick MQ","author_orcid":"0000-0002-8620-827X"},{"name":"Fidock DAF","author_orcid":"0000-0001-6753-8938"},{"name":"Mancia FM","author_orcid":"0000-0003-3293-2200"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure and drug resistance of the Plasmodium falciparum transporter PfCRT","volume":"576","country":"United Kingdom","first_page":"315","last_page":"320","year":"2019","language":"English","doi":"10.1038/s41586-019-1795-x","pubmedid":"31776516","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7786","preprint":false}],"dataset_size":"830.4 GB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6ukj"],"entry_doi":"10.6019/EMPIAR-10330"}}