{"EMPIAR-10321":{"imagesets":[{"segmentations":[],"name":"cryo-ET of cryo-FIB milled yeast cell with high intracellular calcium (scs2/22 ist2 deletion and GCaMP expression)","directory":"data/Tiltseries/TS_frames_aligned_reordered","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":121,"frames_per_image":4,"frame_range_min":1,"frame_range_max":4,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":3.753,"pixel_height":3.753,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The cells were treated with 200 mM calcium chloride directly before plunge freezing. Cells with high GCaMP fluorescence by cryo-FM were targeted for cryo-FIB milling. Electron cryo-tomographic tilt-series were collected on a Titan Krios (FEI) operated at 300 kV using a Quantum energy filter (slit width 20 eV) and a K2 direct electron detector (Gatan) in counting mode at a pixel size of 3.7 angstroms and at a dose rate of ~ 2-4 e-/pixel/second on the detector. Tilt-series were acquired between +/- 60 degrees starting from 0 degrees with 1 degrees increment using SerialEM (Mastronarde, 2005) following a grouped dose-symmetric acquisition with a group size of 4 (Bharat et al., 2018; Hagen et al., 2017), and at -5 micron defocus. A dose of 1.0 e-/square angstroms was applied per image of the tilt-series. The tilt-series frames were aligned and then reordered by tilt angle using IMOD.","image_width":"3710","image_height":"3838"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"cryo-ET of cryo-FIB milled yeast cell in which scs2/22 ist2 are deleted with high intracellular calcium","principal_investigator":[{"author_orcid":"0000-0002-2778-3936","middle_name":null,"organization":"Cell Biology Division, MRC Laboratory of Molecular Biology","street":"Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Wanda","last_name":"Kukulski","email":"kukulski [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10321"}],"status":"REL","deposition_date":"2019-09-23","release_date":"2019-11-18","obsolete_date":null,"update_date":"2019-11-18","corresponding_author":{"author":{"author_orcid":"0000-0002-2778-3936","middle_name":null,"organization":"Cell Biology Division, MRC Laboratory of Molecular Biology","street":"Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Wanda","last_name":"Kukulski","country":"United Kingdom"}},"authors":[{"author":{"name":"Hoffmann PC","author_orcid":"0000-0003-3421-6363"}},{"author":{"name":"Bharat TAM","author_orcid":"0000-0002-0168-0277"}},{"author":{"name":"Wozny MR","author_orcid":"0000-0001-9359-3287"}},{"author":{"name":"Boulanger J","author_orcid":"0000-0003-0237-3743"}},{"author":{"name":"Miller EA","author_orcid":"0000-0002-1033-8369"}},{"author":{"name":"Kukulski W","author_orcid":"0000-0002-2778-3936"}}],"cross_references":["EMD-10309"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hoffmann PC","author_orcid":"0000-0003-3421-6363"},{"name":"Bharat TAM","author_orcid":"0000-0002-0168-0277"},{"name":"Wozny MR","author_orcid":"0000-0001-9359-3287"},{"name":"Boulanger J","author_orcid":"0000-0003-0237-3743"},{"name":"Miller EA","author_orcid":"0000-0002-1033-8369"},{"name":"Kukulski W","author_orcid":"0000-0002-2778-3936"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Tricalbins Contribute to Cellular Lipid Flux and Form Curved ER-PM Contacts that Are Bridged by Rod-Shaped Structures","volume":"51","country":"","first_page":null,"last_page":null,"year":"2019","language":null,"doi":"10.1016/j.devcel.2019.09.019","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Developmental Cell","journal_abbreviation":"Dev Cell","issue":"4","preprint":false}],"dataset_size":"14.3 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10321"}}