{"EMPIAR-10303":{"imagesets":[{"segmentations":[],"name":"Unaligned movies for E2P class 1,2,3","directory":"data/Movies/Dataset0","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6257,"frames_per_image":62,"frame_range_min":1,"frame_range_max":62,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Movies/Dataset0/*.tif","picked_particles_file_pattern":"data/Particles/dataset0-all.star","picked_particles_directory":"data/Particles/Dataset0","details":"Images are gain-corrected.  Note that we started processing with the nominal pixel size of 0.85 Å (as shown in the tiff header) and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.83 Å.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movies for E2Pi-PL and E1P","directory":"data/Movies/Dataset1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":6843,"frames_per_image":54,"frame_range_min":1,"frame_range_max":54,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Movies/Dataset1/*.tif","picked_particles_file_pattern":"data/Particles/dataset1_*-all.star","picked_particles_directory":"data/Particles/Dataset1_*","details":"Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movies for E1P-ADP","directory":"data/Movies/Dataset2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4401,"frames_per_image":54,"frame_range_min":1,"frame_range_max":54,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Movies/Dataset2/*.tif","picked_particles_file_pattern":"data/Particles/dataset2-all.star","picked_particles_directory":"data/Particles/Dataset2","details":"Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movies for E1 class1,2,3","directory":"data/Movies/Dataset3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3590,"frames_per_image":54,"frame_range_min":1,"frame_range_max":54,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Movies/Dataset3/*.tif","picked_particles_file_pattern":"data/Particles/dataset3-all.star","picked_particles_directory":"data/Particles/Dataset3","details":"Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movies for E1-ATP class 1,2,3","directory":"data/Movies/Dataset4","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4333,"frames_per_image":54,"frame_range_min":1,"frame_range_max":54,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Movies/Dataset4/*.tif","picked_particles_file_pattern":"data/Particles/dataset4-all.star","picked_particles_directory":"data/Particles/Dataset4","details":"Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structures of human P4-ATPase flippase","principal_investigator":[{"author_orcid":"0000-0003-1813-7008","middle_name":null,"organization":"Department of Biological Sciences, Graduate School of Science, The University of Tokyo","street":"7-3-1 Hongo","town_or_city":"Bunkyoku","state_or_province":"Tokyo","post_or_zip":"113-0033","telephone":null,"fax":null,"first_name":"Osamu","last_name":"Nureki","email":"nureki [at] bs.s.u-tokyo.ac.jp","country":"Japan","entry":"EMPIAR-10303"}],"status":"REL","deposition_date":"2019-07-22","release_date":"2019-08-30","obsolete_date":null,"update_date":"2019-08-30","corresponding_author":{"author":{"author_orcid":"0000-0001-7463-8398","middle_name":null,"organization":"Department of Biological Sciences, Graduate School of Science, The University of Tokyo","street":"7-3-1 Hongo","town_or_city":"Bunkyoku","state_or_province":"Tokyo","post_or_zip":"113-0033","first_name":"Tomohiro","last_name":"Nishizawa","country":"Japan"}},"authors":[{"author":{"name":"Hiraizumi M","author_orcid":"0000-0002-4340-2937"}},{"author":{"name":"Yamashita K","author_orcid":"0000-0002-5442-7582"}},{"author":{"name":"Nishizawa T","author_orcid":"0000-0001-7463-8398"}},{"author":{"name":"Nureki O","author_orcid":"0000-0003-1813-7008"}}],"cross_references":["EMD-9934","EMD-9942","EMD-9936","EMD-9932","EMD-9941","EMD-9937","EMD-9931","EMD-9939","EMD-9935","EMD-9933"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hiraizumi M","author_orcid":"0000-0002-4340-2937"},{"name":"Yamashita K","author_orcid":"0000-0002-5442-7582"},{"name":"Nisihzawa T","author_orcid":"0000-0001-7463-8398"},{"name":"Nureki O","author_orcid":"0000-0003-1813-7008"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM structures capture the transport cycle of the P4-ATPase flippase","volume":null,"country":"","first_page":null,"last_page":null,"year":"2019","language":"English","doi":"10.1126/science.aay3353","pubmedid":"31416931","details":"Movies were collected on Titan Krios G3i with Gatan K3. The calibrated pixel size for all datasets is 0.83 A. Five datasets were used to yield the structures.\n  \nDataset 0: EMD-9938, EMD-9939, EMD-9940 (E2P class 1,2,3)\nDataset 1: EMD-9941 (E2Pi-PL), EMD-9942 (E1P)\nDataset 2: EMD-9937 (E1P-ADP)\nDataset 3: EMD-9931, EMD-9932, EMD-9933 (E1 class 1,2,3)\nDataset 4: EMD-9935, EMD-9934, EMD-9936 (E1-ATP class 1,2,3)\n\nDose rate (shown on SerialEM) is ~1 e/A2/frame.\nDataset 0 images are gain-corrected. Gain references for datasets 1-4 are provided, which need to be flipped ('Flip upside down (1)' in RELION).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":null,"preprint":false}],"dataset_size":"11.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6k7i","6k7n","6k7h","6k7m","6k7k","6k7g","6k7l","6k7j"],"entry_doi":"10.6019/EMPIAR-10303"}}