{"EMPIAR-10298":{"imagesets":[{"segmentations":[],"name":"Unaligned movies of P-complex spliceosome at 105,000x magnification (Dataset 1)","directory":"data/Movies_7Oct","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2384,"frames_per_image":20,"frame_range_min":1,"frame_range_max":20,"voxel_type":"32 BIT FLOAT","pixel_width":1.12,"pixel_height":1.12,"micrographs_file_pattern":"data/Movies_7Oct/GridSquare_*/Data/FoilHole_*_Data_*_*_201710*_*-*.mrc","picked_particles_file_pattern":"data/mergeddata_polished_refined_data.star","picked_particles_directory":"data/","details":"Unaligned, gain-corrected, 20-frame movies from K2 in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 47 electrons per square Angstrom over 20 frames (12 s). Nominal pixel size is 1.12 Å/pixel, this is uncalibrated. This is the full dataset, so several \"bad\" micrographs will be present - during data processing, we discarded 89 micrographs that had ice contamination or power spectra.\n\nThe attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Unaligned movies of P-complex spliceosome at 130,000x magnification (Dataset 2)","directory":"data/Movies_23Dec","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1614,"frames_per_image":35,"frame_range_min":1,"frame_range_max":35,"voxel_type":"32 BIT FLOAT","pixel_width":0.88,"pixel_height":0.88,"micrographs_file_pattern":"data/Movies_23Dec/GridSquare_*/Data/FoilHole_*_Data_*_*_201712*_*-*.mrc","picked_particles_file_pattern":"data/","picked_particles_directory":"data/mergeddata_polished_refined_data.star","details":"Unaligned, gain-corrected, 35-frame movies from K2. 173 are in super-resolution mode, 1441 are in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 45 electrons per square Angstrom over 35 frames (7 s). Pixel size is 0.88 Å/pixel, calibrated by correlating the calculated map to the map from Dataset 1 using a reference pixel size of 1.12 Å. This is the full dataset, so several \"bad\" micrographs will be present. The super-resolution micrographs are not helpfully separated from the counting micrographs, I suggest during processing to perform a command like \"find data/Movies_23D/ -size +3G\" to separate the super-resolution micrographs.\n\nThe attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph. The X and Y coordinates for the super-resolution micrographs refer to 2x binned micrographs.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Yeast postcatalytic spliceosome, two cryoEM data sets at different magnifications","principal_investigator":[{"author_orcid":"0000-0003-1785-6510","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Kiyoshi","last_name":"Nagai","email":"kn [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-10298"}],"status":"REL","deposition_date":"2019-07-23","release_date":"2019-08-27","obsolete_date":null,"update_date":"2021-02-12","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"MRC Laboratory of Molecular Biology; University of Cambridge","street":"Francis Crick Avenue","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Max","last_name":"Wilkinson","country":"United Kingdom"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Nagai K","author_orcid":"0000-0003-1785-6510"}}],"cross_references":["EMD-10140"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Kumar A","author_orcid":"0000-0002-6366-5451"},{"name":"Casañal A","author_orcid":"0000-0002-0334-0591"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Methods for merging data sets in electron cryo-microscopy","volume":"75","country":"","first_page":"782","last_page":"791","year":"2019","language":"English","doi":"10.1107/s2059798319010519","pubmedid":"31478901","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Acta crystallographica. Section D, Structural biology","journal_abbreviation":"Acta Crystallogr D Struct Biol","issue":"Pt 9","preprint":false}],"dataset_size":"6.3 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10298"}}