{"EMPIAR-10285":{"imagesets":[{"segmentations":[],"name":"Movie files (.tif) for P-Rex1-Gbg","directory":"data/movies","category":"micrographs - multiframe","header_format":"MRCS","data_format":"TIFF","num_images_or_tilt_series":6746,"frames_per_image":30,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":null,"image_height":null},{"segmentations":[],"name":"Micrograph files (.mrc) & CTF log files for P-Rex1-Gbg","directory":"data/average","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6736,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":null,"image_height":null},{"segmentations":[],"name":"Extracted particles from Warp for P-Rex1-Gbg","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":6360,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"data/movies/*.tif","picked_particles_file_pattern":"data/particles/*.mrcs","picked_particles_directory":"data/particles","details":"Particle coordinate files (*.star) for P-Rex1-Gbg can be found in the directory data/matching","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron microscopy structure of the P-Rex1–G-beta-gamma signaling scaffold","principal_investigator":[{"author_orcid":"0000-0002-2067-4999","middle_name":null,"organization":"Department of Biological Chemistry, Life Sciences Institute, University of Michigan","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Michael","last_name":"Cianfrocco","email":"mcianfro [at] umich.edu","country":"United States","entry":"EMPIAR-10285"}],"status":"REL","deposition_date":"2019-06-18","release_date":"2019-11-19","obsolete_date":null,"update_date":"2019-11-19","corresponding_author":{"author":{"author_orcid":"0000-0002-2067-4999","middle_name":null,"organization":"Department of Biological Chemistry, Life Sciences Institute, University of Michigan","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"MI","post_or_zip":"48109","first_name":"Michael","last_name":"Cianfrocco","country":"United States"}},"authors":[{"author":{"name":"Cash JN","author_orcid":"0000-0002-0277-7652"}},{"author":{"name":"Urata S","author_orcid":"0000-0001-7512-4081"}},{"author":{"name":"Li S","author_orcid":"0000-0002-9073-6809"}},{"author":{"name":"Ravala SK","author_orcid":null}},{"author":{"name":"Avramova LV","author_orcid":null}},{"author":{"name":"Shost MD","author_orcid":"0000-0003-1833-0342"}},{"author":{"name":"Gutkind JS","author_orcid":"0000-0002-5150-4482"}},{"author":{"name":"Tesmer JJG","author_orcid":"0000-0003-1125-3727"}},{"author":{"name":"Cianfrocco MA","author_orcid":"0000-0002-2067-4999"}}],"cross_references":["EMD-20308"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Cash JN","author_orcid":"0000-0002-0277-7652"},{"name":"Urata S","author_orcid":"0000-0001-7512-4081"},{"name":"Li S","author_orcid":"0000-0002-9073-6809"},{"name":"Ravala SK","author_orcid":null},{"name":"Avramova LV","author_orcid":null},{"name":"Shost MD","author_orcid":"0000-0003-1833-0342"},{"name":"Gutkind JS","author_orcid":"0000-0002-5150-4482"},{"name":"Tesmer JJG","author_orcid":"0000-0003-1125-3727"},{"name":"Cianfrocco MA","author_orcid":"0000-0002-2067-4999"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-electron microscopy structure and analysis of the P-Rex1-Gβγ signaling scaffold","volume":"5","country":"","first_page":null,"last_page":null,"year":"2019","language":"English","doi":"10.1126/sciadv.aax8855","pubmedid":"31663027","details":"Raw movies (*.tif), micrographs (averages/*.mrc), particle coordinates (matching/*.star), and extracted stack (particles/*.mrcs) for P-Rex-Gbg cryo-EM structure. All data with prefix \"18jan18c\" were collected on an untilted grid whereas data with the prefix \"18aug03b\" were collected on a grid with a tilt angle of 30 degrees. Uploaded data were preprocessed using Warp-1.0.5.\n\nOriginal particle stack (905,464 particles): merge_18aug03b_18jan18c_goodparticles_Retrain1_BoxNet2Mask_20180531_2.star\nSelected stack after 2D classification (611,231 particles): cryosparc_exp001001_202.star \nFinal stack after 3D heterogeneous refinement (205,599 particles): cryosparc_exp001054_009.star\nWarp re-trained model: Retrain1_BoxNet2Mask_20180531_2/\nGain reference for 18jan18c: 18jan18c_gain_reference_17133354_01_7420x7676_norm_0.mrc\nGain reference for 18aug03b: 18aug03b_gain_reference_02131216_05_3710x3838_norm_0.mrc\n\nKev=300\nCs=2.7\nPixel size=1A/pix\nUntilted (18jan18c) total dose: 49.5 e/A2 (30 frames)\nTilted (18aug03b) total dose: 45 e/A2 (100 frames)","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"10","preprint":false}],"dataset_size":"3.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["6pcv"],"entry_doi":"10.6019/EMPIAR-10285"}}