{"EMPIAR-10252":{"imagesets":[{"segmentations":[],"name":"Raw, unaligned movie stacks of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 acquired on a Talos Arctica using a K2 direct electron detector","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":4812,"frames_per_image":44,"frame_range_min":0,"frame_range_max":43,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.5585,"pixel_height":0.5585,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw, unaligned counting mode frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Three separate data collections – 18feb27c (collected without tilt), 18mar03b (collected without tilt), and 18aug21a (collected at a 30˚ tilt) – that were combined are provided. Images used for corrections are also provided (require flip in Y).","image_width":"3710","image_height":"3838"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 movies obtained using Talos Arctica operating at 200 kV equipped with a K2","principal_investigator":[{"author_orcid":"0000-0003-4921-1135","middle_name":"C","organization":"The Scripps Research Institute","street":"10550 N. Torrey Pines Rd","town_or_city":"La Jolla","state_or_province":"CA","post_or_zip":"92037","telephone":"858-784-9499","fax":null,"first_name":"Gabriel","last_name":"Lander","email":"glander [at] scripps.edu","country":"United States","entry":"EMPIAR-10252"}],"status":"REL","deposition_date":"2019-02-11","release_date":"2019-03-25","obsolete_date":null,"update_date":"2019-04-12","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"Anthony","organization":"The Scripps Research Institute","street":"10550 N. Torrey Pines Rd","town_or_city":"La Jolla","state_or_province":"CA","post_or_zip":"92037","first_name":"Mark","last_name":"Herzik Jr.","country":"United States"}},"authors":[{"author":{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}},{"author":{"name":"Wu M","author_orcid":"0000-0002-2248-9383"}},{"author":{"name":"Lander GC","author_orcid":"0000-0003-4921-1135"}}],"cross_references":["EMD-0409"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"},{"name":"Wu M","author_orcid":"0000-0002-2248-9383"},{"name":"Lander GC","author_orcid":"0000-0003-4921-1135"}],"editors":[{"name":"Lander GC","author_orcid":null},{"name":"Wu M","author_orcid":null},{"name":"Herzik Jr MA","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM","volume":null,"country":"","first_page":null,"last_page":null,"year":"2018","language":"English","doi":"10.1101/489898","pubmedid":null,"details":"Raw, unaligned frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and protein kinase inhibitor (IP20) in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector operating in counting mode - stage position used for exposure navigation. Movies were acquired in counting mode over 11 s with an exposure rate of 1.95 electrons per pixel per second, yielding a total exposure of ~69 electrons per squared Å (1.57 electrons per squared Å per frame), and a nominal defocus range of -0.6 μm to -1.8 μm. Data were collected over three separate sessions – two sessions without tilting the specimen (18feb27c and 18mar03b) and one session while tilting the specimen 30˚ (18aug21a). Images used for normalizations are also included (require flip in Y axis).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"5.5 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10252"}}