{"EMPIAR-10155":{"imagesets":[{"segmentations":[],"name":"Unaligned K2 DED image tilt series of yeast NPC and associated files","directory":"data/tiltseries","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":120,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":5.6,"pixel_height":5.6,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"Data collected with UCSF Tomo and best fit value for pixel size is 5.3 Angstroms. Variable number of tilts in the tilt series, due to different sampling and some higher tilted images with bad drift were removed. Additional files are provided , adoc parameter files used\nin IMOD Batchtomo, list files with nominal and fitted tilt angles and\nfitted defocus values. Coordinate files for particles selected with\ne2spt_boxer.py are also given, a box size of 300 was used for\nfinal processing with unbinned data.","image_width":"3710","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron tomography of the yeast NPC","principal_investigator":[{"author_orcid":"0000-0002-3059-3121","middle_name":"Winslow","organization":"Department of Physiology and Biophysics, Boston University School of Medicine","street":"700 Albany Street","town_or_city":"Boston","state_or_province":"Massachusetts","post_or_zip":"02118","telephone":null,"fax":null,"first_name":"Christopher","last_name":"Akey","email":"cakey [at] bu.edu","country":"United States","entry":"EMPIAR-10155"},{"author_orcid":null,"middle_name":"J","organization":"National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","street":"1 Baylor Plaza","town_or_city":"Houston","state_or_province":"Texas","post_or_zip":"77030","telephone":null,"fax":null,"first_name":"Steven","last_name":"Ludtke","email":"sludtke [at] bcm.edu","country":"United States","entry":"EMPIAR-10155"},{"author_orcid":null,"middle_name":"P","organization":"Laboratory of Cellular and Structural Biology, Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Michael","last_name":"Rout","email":"rout [at] rockefeller.edu","country":"United States","entry":"EMPIAR-10155"}],"status":"REL","deposition_date":"2018-01-18","release_date":"2018-03-20","obsolete_date":null,"update_date":"2018-03-20","corresponding_author":{"author":{"author_orcid":"0000-0002-3059-3121","middle_name":"Winslow","organization":"Department of Physiology and Biophysics, Boston University School of Medicine","street":"700 Albany Street","town_or_city":"Boston","state_or_province":"Massachusetts","post_or_zip":"02118","first_name":"Christopher","last_name":"Akey","country":"United States"}},"authors":[{"author":{"name":"Nudelman I","author_orcid":null}},{"author":{"name":"Fernandez-Martinez J","author_orcid":null}},{"author":{"name":"Rout MP","author_orcid":null}},{"author":{"name":"Ludtke SJ","author_orcid":null}},{"author":{"name":"Akey CW","author_orcid":null}}],"cross_references":["EMD-7321"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kim SJ","author_orcid":null},{"name":"Fernandez-Martinez J","author_orcid":null},{"name":"Nudelman I","author_orcid":null},{"name":"Shi Y","author_orcid":null},{"name":"Zhang W","author_orcid":null},{"name":"Raveh B","author_orcid":null},{"name":"Herricks T","author_orcid":null},{"name":"Slaughter BD","author_orcid":null},{"name":"Hogan J","author_orcid":null},{"name":"Upla P","author_orcid":null},{"name":"Chemmama IE","author_orcid":null},{"name":"Pellarin R","author_orcid":null},{"name":"Echeverria I","author_orcid":null},{"name":"Shivaraju M","author_orcid":null},{"name":"Chaudhury AS","author_orcid":null},{"name":"Wang J","author_orcid":null},{"name":"Williams R","author_orcid":null},{"name":"Unruh JR","author_orcid":null},{"name":"Greenberg CH","author_orcid":null},{"name":"Jacobs EY","author_orcid":null},{"name":"Yu Z","author_orcid":null},{"name":"Jason de la Cruz M","author_orcid":null},{"name":"Mironska R","author_orcid":null},{"name":"Stokes DL","author_orcid":null},{"name":"Aitchison JD","author_orcid":null},{"name":"Jarrold MF","author_orcid":null},{"name":"Gerton JL","author_orcid":null},{"name":"Ludtke SJ","author_orcid":null},{"name":"Akey CW","author_orcid":null},{"name":"Chait BT","author_orcid":null},{"name":"Sali A","author_orcid":null},{"name":"Rout MP","author_orcid":null},{"name":"Hogan JA","author_orcid":null},{"name":"de la Cruz MJ","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Integrative structure and functional anatomy of a nuclear pore complex","volume":null,"country":"United Kingdom","first_page":null,"last_page":null,"year":"2018","language":"English","doi":"10.1038/nature26003","pubmedid":"29539637","details":"An integrative structure with subnanometer precision was determined for the entire 552-protein yeast NPC by satisfying diverse data, including  a 3D cryo-EM map obtained by averaging sub-volumes from  tomograms, stoichiometry measurements, and chemical cross-links. \n\n","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"127.1 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10155"}}