{"EMPIAR-10130":{"imagesets":[{"segmentations":[],"name":"Super-resolution K2 tilt-series frames","directory":"data/frames","category":"micrographs - multiframe","header_format":"MRCS","data_format":"MRC","num_images_or_tilt_series":60,"frames_per_image":5,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.5595,"pixel_height":0.5595,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"- 1 mg/ml rabbit muscle aldolase on a gold Spotiton nanowire grid,\n- Collected bi-directionally from [-12:45] then [-12:-45] with 3 degree tilt increment,\n- Collected ~50 nm away from the edge of 1-2 micron diameter holes because farther out there are no particles,\n- Ice is about 15-25 nm thin,\n- Collected with a random defocus range between 1.2 and 2.2 microns,\n- Collected at 105kx mag = 1.119 angstrom pixelsize, 0.5595 angstrom pixelsize at super-res (calibrated: 0.530245),\n- 15-20 eV slit at ZLP (I think most was 15 eV, I don't remember), re-aligned automatically every 1.25 hours,\n- ~8 e-/px/s dose rate on the camera,\n- 1.54 e-/A^2 for tilt angles up to 24 degrees, 1.84 e-/A^2 for 27 to 39 degrees, 2.15 e-/A^2 for 42 and 45 degrees,\n- 100 ms frames,\n- 5-7 frames per tilt image, depending on tilt (cosine increase in dose),\n- ~< 1 angstrom per-image drift at all tilt angles,\n- tiltseries0003 and tiltseries0004 were collected without the objective aperture in.\n- Gain and dark references included.","image_width":"7420","image_height":"7676"},{"segmentations":[],"name":"Whole-frame aligned tilt images along with all other magnification images from the collection","directory":"data/leginon","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":60,"frames_per_image":5,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.5595,"pixel_height":0.5595,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Whole-frame aligned tilt images using Motioncor2.\nCalibrated pixelsize: 0.530245","image_width":"7420","image_height":"7676"},{"segmentations":[],"name":"Appion-Protomo tilt-series alignments","directory":"data/protomo_alignments","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":30,"frames_per_image":5,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.5595,"pixel_height":0.5595,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Includes a full tilt-series alignment run for 30 of the ~50 good tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.\nCalibrated pixelsize: 0.530245","image_width":"7420","image_height":"7676"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"CryoET of rabbit muscle aldolase single particle super-res","principal_investigator":[{"author_orcid":"0000-0002-0624-5020","middle_name":null,"organization":"New York Structural Biology Center","street":"89 Convent Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Bridget","last_name":"Carragher","email":"bcarr [at] nysbc.org","country":"United States","entry":"EMPIAR-10130"}],"status":"REL","deposition_date":"2017-12-05","release_date":"2017-12-15","obsolete_date":null,"update_date":"2022-07-14","corresponding_author":{"author":{"author_orcid":"0000-0002-0624-5020","middle_name":null,"organization":"New York Structural Biology Center","street":"89 Convent Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","first_name":"Bridget","last_name":"Carragher","country":"United States"}},"authors":[{"author":{"name":"Noble AJ","author_orcid":"0000-0001-8634-2279"}},{"author":{"name":"Dandey VP","author_orcid":null}},{"author":{"name":"Wei H","author_orcid":null}},{"author":{"name":"Brasch J","author_orcid":null}},{"author":{"name":"Chase J","author_orcid":null}},{"author":{"name":"Acharya P","author_orcid":null}},{"author":{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Zhang Z","author_orcid":null}},{"author":{"name":"Kim LY","author_orcid":null}},{"author":{"name":"Scapin G","author_orcid":null}},{"author":{"name":"Rapp M","author_orcid":null}},{"author":{"name":"Eng ET","author_orcid":"0000-0002-8014-7269"}},{"author":{"name":"Rice WJ","author_orcid":null}},{"author":{"name":"Cheng A","author_orcid":null}},{"author":{"name":"Negro CJ","author_orcid":null}},{"author":{"name":"Shapiro L","author_orcid":null}},{"author":{"name":"Kwong PD","author_orcid":null}},{"author":{"name":"Jeruzalmi D","author_orcid":null}},{"author":{"name":"des Georges A","author_orcid":null}},{"author":{"name":"Potter CS","author_orcid":null}},{"author":{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"}}],"cross_references":["EMD-7138"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Noble AJ","author_orcid":"0000-0001-8634-2279"},{"name":"Dandey VP","author_orcid":null},{"name":"Wei H","author_orcid":null},{"name":"Brasch J","author_orcid":null},{"name":"Chase J","author_orcid":null},{"name":"Acharya P","author_orcid":null},{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"},{"name":"Zhang Z","author_orcid":null},{"name":"Kim LY","author_orcid":null},{"name":"Scapin G","author_orcid":null},{"name":"Rapp M","author_orcid":null},{"name":"Eng ET","author_orcid":"0000-0002-8014-7269"},{"name":"Rice WJ","author_orcid":null},{"name":"Cheng A","author_orcid":null},{"name":"Negro CJ","author_orcid":null},{"name":"Shapiro L","author_orcid":null},{"name":"Kwong PD","author_orcid":null},{"name":"Jeruzalmi D","author_orcid":"0000-0001-5886-1370"},{"name":"des Georges A","author_orcid":null},{"name":"Potter CS","author_orcid":"0000-0003-2394-0831"},{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Routine single particle CryoEM sample and grid characterization by tomography","volume":"7","country":"","first_page":null,"last_page":null,"year":"2018","language":"English","doi":"10.7554/elife.34257","pubmedid":"29809143","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"998.3 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10130"}}