{"EMPIAR-10114":{"imagesets":[{"segmentations":[],"name":"Tilt-series for e. coli carrying the ple7 plasmid carrying YFP-MreB induced with 20 uM IPTG","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7,"frames_per_image":122,"frame_range_min":1,"frame_range_max":122,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"","image_width":"2032","image_height":"2032"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Tilt-series of e. coli carrying the ple7 plasmid carrying YFP-MreB induced with 20 uM IPTG","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"Division of Biology, California Institute of Technology","street":"1200 E California Blvd","town_or_city":"Pasadena","state_or_province":"California","post_or_zip":"91125","telephone":"16263958827","fax":null,"first_name":"Grant","last_name":"Jensen","email":"jensen [at] caltech.edu","country":"United States","entry":"EMPIAR-10114"}],"status":"REL","deposition_date":"2017-07-04","release_date":"2018-01-24","obsolete_date":null,"update_date":"2018-01-24","corresponding_author":{"author":{"author_orcid":null,"middle_name":null,"organization":"Division of Biology, California Institute of Technology","street":"1200 E California Blvd","town_or_city":"Pasadena","state_or_province":"California","post_or_zip":"91125","first_name":"Matthew","last_name":"Swulius","country":"United States"}},"authors":[{"author":{"name":"Swulius MT","author_orcid":null}},{"author":{"name":"Jensen GJ","author_orcid":null}}],"cross_references":["EMD-3811"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Swulius MT","author_orcid":null},{"name":"Jensen GJ","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag","volume":"194","country":"","first_page":"6382","last_page":"6386","year":"2012","language":"English","doi":"10.1128/jb.00505-12","pubmedid":"22904287","details":"Based on fluorescence microscopy, the actin homolog MreB has been thought to form extended helices surrounding the cytoplasm of rod-shaped bacterial cells. The presence of these and other putative helices has come to dominate models of bacterial cell shape regulation, chromosome segregation, polarity, and motility. In the publication electron cryotomography was used to show that MreB does in fact form extended helices and filaments in Escherichia coli when yellow fluorescent protein (YFP) is fused to its N terminus but native (untagged) MreB expressed to the same levels does not. In contrast, mCherry fused to an internal loop (MreBRFPSW) does not induce helices. The helices are therefore an artifact of the placement of the fluorescent protein tag. YFP-MreB helices were also clearly distinguishable from the punctate, “patchy” localization patterns of MreB-RFPSW, even by standard light microscopy. The many interpretations in the literature of such punctate patterns as helices should therefore be reconsidered.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of bacteriology","journal_abbreviation":"J. Bacteriol.","issue":"23","preprint":false}],"dataset_size":"6.6 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10114"}}