{"EMPIAR-10111":{"imagesets":[{"segmentations":[],"name":"Tilt-series for salmonella enterica wild-type bacterial flagellar motor","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"Tilt series consist of 41 images.","image_width":"2048","image_height":"2048"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Tilt-series of salmonella enterica wild-type bacterial flagellar motor","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"Division of Biology, California Institute of Technology","street":"1200 E California Blvd","town_or_city":"Pasadena","state_or_province":"California","post_or_zip":"91125","telephone":"16263958827","fax":null,"first_name":"Grant","last_name":"Jensen","email":"jensen [at] caltech.edu","country":"United States","entry":"EMPIAR-10111"}],"status":"REL","deposition_date":"2017-06-30","release_date":"2018-02-08","obsolete_date":null,"update_date":"2018-02-08","corresponding_author":{"author":{"author_orcid":"0000-0001-6413-9835","middle_name":null,"organization":"Department of Life Sciences, Imperial College of London","street":null,"town_or_city":null,"state_or_province":null,"post_or_zip":"SW7 2AZ","first_name":"Morgan","last_name":"Beeby","country":"United Kingdom"}},"authors":[{"author":{"name":"Beeby M","author_orcid":"0000-0001-6413-9835"}},{"author":{"name":"Ribardo DA","author_orcid":null}},{"author":{"name":"Brennan CA","author_orcid":"0000-0002-6074-9502"}},{"author":{"name":"Ruby EG","author_orcid":null}},{"author":{"name":"Jensen GJ","author_orcid":null}},{"author":{"name":"Hendrixson DR","author_orcid":null}}],"cross_references":["EMD-3813","EMD-3154"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Beeby M","author_orcid":"0000-0001-6413-9835"},{"name":"Ribardo DA","author_orcid":null},{"name":"Brennan CA","author_orcid":"0000-0002-6074-9502"},{"name":"Ruby EG","author_orcid":null},{"name":"Jensen GJ","author_orcid":null},{"name":"Hendrixson DR","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold","volume":"113","country":"United States","first_page":"E1917","last_page":"E1926","year":"2016","language":"English","doi":"10.1073/pnas.1518952113","pubmedid":"26976588","details":"Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference genetic analyses and electron cryo-tomography was combined to determine in situ structures of flagellar motors that produce different torques, from Campylobacter and Vibrio species.The results unambiguously locate the torque generating stator complexes and show that diverse high-torque motors use variants of an ancestrally related family of structures to scaffold incorporation of additional stator complexes at wider radii from the axial driveshaft than in the model enteric motor. The protein components were identified for the additional scaffold structures and their sequential assembly was elucidated, demonstrating that they are required for stator-complex incorporation. These proteins are widespread, suggesting that different bacteria have tailored torques to specific environments by scaffolding alternative stator placement and number. The results quantitatively account for different motor torques, complete the assignment of the locations of the major flagellar components, and provide crucial constraints for understanding mechanisms of torque generation and the evolution of multiprotein complexes.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc. Natl. Acad. Sci. U.S.A.","issue":"13","preprint":false}],"dataset_size":"328.0 MB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10111"}}