{"EMPIAR-10105":{"imagesets":[{"segmentations":[],"name":"FIB-SEM images of a dividing cell at 5.7 min after anaphase onset","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2620,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":5.0,"pixel_height":8.0,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the first half of the cell for segmentation. ","image_width":"4190","image_height":"6164"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"FIB-SEM of a dividing cell at 5.7 min after anaphase onset","principal_investigator":[{"author_orcid":"0000-0001-5909-701X","middle_name":null,"organization":"European Molecular Biology Laboratory","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69117","telephone":null,"fax":null,"first_name":"Jan","last_name":"Ellenberg","email":"jan.ellenberg [at] embl.de","country":"Germany","entry":"EMPIAR-10105"}],"status":"REL","deposition_date":"2017-05-25","release_date":"2017-11-28","obsolete_date":null,"update_date":"2021-10-25","corresponding_author":{"author":{"author_orcid":"0000-0001-5909-701X","middle_name":null,"organization":"European Molecular Biology Laboratory","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":null,"post_or_zip":"69117","first_name":"Jan","last_name":"Ellenberg","country":"Germany"}},"authors":[{"author":{"name":"Otsuka S","author_orcid":"0000-0003-3976-0843"}},{"author":{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"}},{"author":{"name":"Schorb M","author_orcid":"0000-0003-4910-1868"}},{"author":{"name":"Hériché JK","author_orcid":"0000-0001-6867-9425"}},{"author":{"name":"Hossain MJ","author_orcid":"0000-0003-3303-5755"}},{"author":{"name":"Sethi S","author_orcid":null}},{"author":{"name":"Kueblbeck M","author_orcid":null}},{"author":{"name":"Schwab Y","author_orcid":null}},{"author":{"name":"Beck M","author_orcid":"0000-0002-7397-1321"}},{"author":{"name":"Ellenberg J","author_orcid":"0000-0001-5909-701X"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Otsuka S","author_orcid":"0000-0003-3976-0843"},{"name":"Steyer AM","author_orcid":"0000-0002-4814-7517"},{"name":"Schorb M","author_orcid":"0000-0003-4910-1868"},{"name":"Hériché JK","author_orcid":"0000-0001-6867-9425"},{"name":"Hossain MJ","author_orcid":"0000-0003-3303-5755"},{"name":"Sethi S","author_orcid":null},{"name":"Kueblbeck M","author_orcid":null},{"name":"Schwab Y","author_orcid":null},{"name":"Beck M","author_orcid":"0000-0002-7397-1321"},{"name":"Ellenberg J","author_orcid":"0000-0001-5909-701X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings","volume":"25","country":"","first_page":"21","last_page":"28","year":"2017","language":"English","doi":"10.1038/s41594-017-0001-9","pubmedid":"29323269","details":"The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm.  The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the first half of the cell for segmentation.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"1","preprint":false}],"dataset_size":"63.0 GB","experiment_type":"FIB-SEM","scale":null,"entry_doi":"10.6019/EMPIAR-10105"}}