{"EMPIAR-10097":{"imagesets":[{"segmentations":[],"name":"Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer","directory":"data/Raw-Frames","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":668,"frames_per_image":100,"frame_range_min":1,"frame_range_max":100,"voxel_type":"32 BIT FLOAT","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were collected when tilt angle is set to -40 degrees. The gain normalization reference images (bright and norm) are included for each of the 3 sessions. No dark gain reference images are present because dark images values are always zero in counting mode camera.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer","directory":"data/Dose-Weighted-Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":668,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were aligned and summed from the raw frames using MotionCor2, with 5 by 5 patch and exposure weighting.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs","directory":"data/Particle-Stack","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":130000,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs. Particles were extracted from aligned, exposured weighted and summed micrographs. In order to compare these particles with a 40 degree tilted dataset, these particles were equalized based on GCTF-estimated per-particle CTF values so a subset of particles with the same defocus spread would be obtained. This equalized stack of 130,000 is the uploaded stack. A Relion .star metadata file is also provided containing the final refined Euler angles.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer","principal_investigator":[{"author_orcid":"0000-0002-8124-7472","middle_name":null,"organization":"Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies","street":"10010 North Torrey Pines Road","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92037","telephone":"+1 858 453 4100","fax":null,"first_name":"Dmitry","last_name":"Lyumkis","email":"dlyumkis [at] salk.edu","country":"United States","entry":"EMPIAR-10097"}],"status":"REL","deposition_date":"2017-05-11","release_date":"2017-08-16","obsolete_date":null,"update_date":"2021-11-02","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","first_name":"Yong Zi","last_name":"Tan","country":"United States"}},"authors":[{"author":{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Lyumkis D","author_orcid":"0000-0002-8124-7472"}}],"cross_references":["EMD-8731"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"},{"name":"Baldwin PR","author_orcid":"0000-0002-4202-4129"},{"name":"Davis JH","author_orcid":"0000-0002-8858-8907"},{"name":"Williamson JR","author_orcid":"0000-0002-8772-468X"},{"name":"Potter CS","author_orcid":"0000-0002-3287-121X"},{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"},{"name":"Lyumkis D","author_orcid":"0000-0002-8124-7472"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Addressing preferred specimen orientation in single-particle cryo-EM through tilting","volume":"14","country":"United States","first_page":"793","last_page":"796","year":"2017","language":"English","doi":"10.1038/nmeth.4347","pubmedid":"28671674","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature methods","journal_abbreviation":"Nat. Methods","issue":"8","preprint":false}],"dataset_size":"1.8 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10097"}}