{"EMPIAR-10096":{"imagesets":[{"segmentations":[],"name":"Unaligned raw multi-frame untilted micrographs of Influenza Hemagglutinin Trimer","directory":"data/Raw-Frames","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":447,"frames_per_image":100,"frame_range_min":1,"frame_range_max":100,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned raw multi-frame untilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were collected when tilt angle is set to zero. The gain normalization reference images (bright and norm) are included for each of the 3 sessions. No dark gain reference images are present because dark images values are always zero in counting mode camera.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned, summed and exposure weighted untilted micrographs of Influenza Hemagglutinin Trimer","directory":"data/Dose-Weighted-Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":447,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned, summed and exposure weighted untilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were aligned and summed from the raw frames using MotionCor2, with 5 by 5 patch and exposure weighting.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Equalized particle stack of Influenza Hemagglutinin Trimer","directory":"data/Particle-Stack","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":130000,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.31,"pixel_height":1.31,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Equalized particle stack of Influenza Hemagglutinin Trimer. Particles were extracted from aligned, exposured weighted and summed micrographs. In order to compare these particles with a 40 degree tilted dataset, these particles were equalized based on GCTF-estimated per-particle CTF values so a subset of particles with the same defocus spread would be obtained. This equalized stack of 130,000 is the uploaded stack. A Relion .star metadata file is also provided containing the final refined Euler angles.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Untilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer","principal_investigator":[{"author_orcid":"0000-0002-8124-7472","middle_name":null,"organization":"Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies","street":"10010 North Torrey Pines Road","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92037","telephone":"+1 858 453 4100","fax":null,"first_name":"Dmitry","last_name":"Lyumkis","email":"dlyumkis [at] salk.edu","country":"United States","entry":"EMPIAR-10096"}],"status":"REL","deposition_date":"2017-05-09","release_date":"2017-05-15","obsolete_date":null,"update_date":"2021-03-19","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"New York Structural Biology Center/Columbia University","street":"89 Convent Ave","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10027","first_name":"Yong Zi","last_name":"Tan","country":"United States"}},"authors":[{"author":{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"}},{"author":{"name":"Lyumkis D","author_orcid":"0000-0002-8124-7472"}}],"cross_references":["EMD-8731"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tan YZ","author_orcid":"0000-0001-6656-6320"},{"name":"Baldwin PR","author_orcid":"0000-0002-4202-4129"},{"name":"Davis JH","author_orcid":"0000-0002-8858-8907"},{"name":"Williamson JR","author_orcid":"0000-0002-8772-468X"},{"name":"Potter CS","author_orcid":"0000-0002-3287-121X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Addressing preferred specimen orientation in single-particle cryo-EM through tilting","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2017","language":"English","doi":"10.1038/nmeth.4347","pubmedid":"28671674","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature methods","journal_abbreviation":"Nat. Methods","issue":null,"preprint":false}],"dataset_size":"1.2 TB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10096"}}