{"EMPIAR-10078":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of 20S proteasome acquired with a Volta phase plate and defocus","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":427,"frames_per_image":24,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.53,"pixel_height":0.53,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"The movies were acquired with SerialEM as dark-subtracted but non-gain-normalized stacks and saved as LZW-compressed TIFFs. The gain reference is in the \"SuperRef_20S_000_Mar28_14.49.23.mrc\" file. \"MotionCor2_patch9_DW1.sh\" is an example shell script for using MotionCor2 to gain normalize, align and dose-weight the movies. \"micrographs_ctf.star\" is a Relion 2.0 .star file containing the CTF fit results from ctffind4 v4.1.5. \"particles.star\" is a Relion 2.0 .star file containing particle coordinates and CTF fit parameters. Other details about the dataset are in \"dataset_parameters.txt\".\nThe data was used for three reconstruction strategies. All frames were used in the Relion particle polishing and Warp approaches. The first two frames were discarded in the MotionCor2 approach.","image_width":"7676","image_height":"7420"}],"workflow_file":null,"grant_references":[],"version_history":[{"version_number":1,"date":"2017-01-03","status_code":"REL","details":"Added the citation information."}],"title":"Volta phase plate with defocus cryo-EM dataset of Thermoplasma acidophilum 20S proteasome","principal_investigator":[{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"Max Planck Institute of Biochemistry","street":"Am Klopferspitz 18","town_or_city":"Martinsried","state_or_province":null,"post_or_zip":"82152","telephone":"+49-89-8578-2651","fax":null,"first_name":"Radostin","last_name":"Danev","email":"danev [at] biochem.mpg.de","country":"Germany","entry":"EMPIAR-10078"}],"status":"REL","deposition_date":"2016-11-03","release_date":"2016-11-10","obsolete_date":null,"update_date":"2016-11-10","corresponding_author":{"author":{"author_orcid":"0000-0001-6406-8993","middle_name":null,"organization":"Max Planck Institute of Biochemistry","street":"Am Klopferspitz 18","town_or_city":"Martinsried","state_or_province":null,"post_or_zip":"82152","first_name":"Radostin","last_name":"Danev","country":"Germany"}},"authors":[{"author":{"name":"Danev R","author_orcid":"0000-0001-6406-8993"}},{"author":{"name":"Tegunov D","author_orcid":"0000-0001-7019-3221"}},{"author":{"name":"Baumeister W","author_orcid":null}}],"cross_references":["EMD-3457","EMD-3456","EMD-3455"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Danev R","author_orcid":"0000-0001-6406-8993"},{"name":"Tegunov D","author_orcid":"0000-0001-7019-3221"},{"name":"Baumeister W","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Using the Volta phase plate with defocus for cryo-EM single particle analysis","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1101/085530","pubmedid":null,"details":"http://biorxiv.org/content/early/2016/11/03/085530","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"bioRxiv","issue":"1","preprint":true}],"dataset_size":"92.5 GB","experiment_type":"EMDB","scale":null,"entry_doi":"10.6019/EMPIAR-10078"}}