{"EMPIAR-10077":{"imagesets":[{"segmentations":[],"name":"Part 1 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complexes","directory":"data/Micrographs_part1","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6147,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Part 2 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex","directory":"data/Micrographs_part2","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":6534,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"ribosomeSelB_particles.mrcs - CTF corrected single particle images of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex","directory":"data","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":969526,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":null,"picked_particles_file_pattern":null,"picked_particles_directory":null,"details":"Particle image stack \"ribosomeSelB_particles.mrcs\":\nCTF corrected particles images (not normalized; total 969.526 particles)\n\nStar-files for particles corresponding to distinct states in \"ribosomeSelB_particles.mrcs\":\n1. \"particles_IC_state.star\": particles of initial complex, E. coli ribosome 70S-fMet-  tRNAfmet-SECIS mRNA complex (IC)\n2. \"particles_IB_state.star\": particles of initial binding state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (IB)\n3. \"particles_CR_state.star\": particles of codon reading state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (CR)\n4. \"particles_GA_state.star\": particles of GTPase activated state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (GA)\n5. \"particles_C_state.star\": particles of classical pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (C)\n6. \"particles_H_state.star\": particles of hybrid pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (H)","image_width":"272","image_height":"272"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"The pathway to GTPase activation of elongation factor SelB on the ribosome","principal_investigator":[{"author_orcid":null,"middle_name":null,"organization":"Max-Planck-Institute for Biophysical Chemistry","street":"Am Fassberg 11","town_or_city":"Goettingen","state_or_province":"Lower Saxony","post_or_zip":"37073","telephone":"+495512011305","fax":null,"first_name":"Stark","last_name":"Holger","email":"hstark1 [at] gwdg.de","country":"Germany","entry":"EMPIAR-10077"}],"status":"REL","deposition_date":"2016-11-01","release_date":"2016-11-24","obsolete_date":null,"update_date":"2016-11-24","corresponding_author":{"author":{"author_orcid":"0000-0002-4609-4052","middle_name":null,"organization":"Max-Planck-Institute for Biophysical Chemistry","street":"Am Fassberg 11","town_or_city":"Göttingen","state_or_province":"Lower Saxony","post_or_zip":"37073","first_name":"Niels","last_name":"Fischer","country":"Germany"}},"authors":[{"author":{"name":"Fischer N","author_orcid":"0000-0002-4609-4052"}},{"author":{"name":"Neumann P","author_orcid":null}},{"author":{"name":"Bock LV","author_orcid":null}},{"author":{"name":"Maracci C","author_orcid":null}},{"author":{"name":"Wang Z","author_orcid":null}},{"author":{"name":"Paleskava A","author_orcid":null}},{"author":{"name":"Konevega AL","author_orcid":null}},{"author":{"name":"Schroeder GF","author_orcid":null}},{"author":{"name":"Grubmueller H","author_orcid":null}},{"author":{"name":"Rodnina MV","author_orcid":null}},{"author":{"name":"Stark H","author_orcid":null}}],"cross_references":["EMD-4126","EMD-4121","EMD-4125","EMD-4124","EMD-4123","EMD-4122"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Neumann P","author_orcid":null},{"name":"Bock LV","author_orcid":null},{"name":"Maracci C","author_orcid":null},{"name":"Wang Z","author_orcid":null},{"name":"Paleskava A","author_orcid":null},{"name":"Konevega AL","author_orcid":null},{"name":"Schroeder GF","author_orcid":null},{"name":"Grubmueller H","author_orcid":null},{"name":"Ficner R","author_orcid":null},{"name":"Rodnina MV","author_orcid":null},{"name":"Stark H","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"The pathway to GTPase activation of elongation factor SelB on the ribosome","volume":null,"country":"","first_page":null,"last_page":null,"year":"2016","language":"English","doi":"10.1038/nature20560","pubmedid":"27842381","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"1.0 TB","experiment_type":"EMDB","scale":null,"related_pdb_entries":["5lzf","5lza","5lze","5lzd","5lzc","5lzb"],"entry_doi":"10.6019/EMPIAR-10077"}}