Tilt pair validation server
Welcome to the PDBe tilt pair validation server!
Tilt-pair validation analysis (Rosenthal
and Henderson, 2003) can be used to assess the accuracy of initial angle assignment in
single-particle processing. To perform this analysis
you need to collect two corresponding sets of particle images - one untilted and the other tilted, then
upload the stacks of images along with a 3D reconstruction
based on the untilted images. This server is based on the Tilt-pair
server developed at MRC National Institute for Medical Research (Wasilewski and Rosenthal, 2014),
and we thank Sebastian Wasilewski and Peter Rosenthal for their help in developing and testing the
current server.
You may upload map files in MRC or CCP4 format, and parameter files (containing Euler angles for
individual particles) in Spider or Frealign format.
We have some test data sets that you can use to try out the service
here. We are still developing the
server and appreciate
your
feedback!
Quick Links
Recent Entries
(Show all)
EMD-26347
[1/82]
Human CST bound to full-length DNA polymerase alpha-primase in a recruitment state
EMD-31925
[2/82]
The motor-nucleosome module of human chromatin remodeling PBAF-nucleosome complex
EMD-13596
[4/82]
Structure of thermostabilised human NTCP in complex with Megabody 91
EMD-13593
[5/82]
Structure of thermostabilised human NTCP in complex with nanobody 87
EMD-31713
[6/82]
Cryo-EM Structure of Camellia sinensis glutamine synthetase CsGSIb inactive Pentamer State I
EMD-32622
[7/82]
Cryo-EM structure of VWF D'D3 dimer (R1136M/E1143M mutant) complexed with D1D2 at 3.29 angstron resolution (2 units)
EMD-25118
[11/82]
Cryo-EM reconstruction of Kinesin-1 and MAP7, bound to the microtubule
EMD-31714
[12/82]
Cryo-EM Structure of Camellia sinensis glutamine synthetase CsGSIb inactive Pentamer State II
EMD-24722
[13/82]
afTMEM16 in C22 lipid nanodiscs with MSP1E3 scaffold protein in the presnece of Ca2+
EMD-26486
[14/82]
70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B
EMD-32760
[15/82]
Cryo-EM structure of human glucose transporter GLUT4 bound to cytochalasin B in lipid nanodiscs
EMD-25218
[17/82]
Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 2)
EMD-31927
[18/82]
The NBL-DBL module of human chromatin remodeling PBAF-nucleosome complex
EMD-32281
[20/82]
Structure of USP14-bound human 26S proteasome in substrate-inhibited state SB_USP14
EMD-32224
[24/82]
Cryo-EM structure of C22:0-CoA bound human very long-chain fatty acid ABC transporter ABCD1
EMD-25219
[25/82]
Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi showing phage bouquets (same as Fig 4A of the manuscript)
EMD-32095
[26/82]
Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs
EMD-33070
[27/82]
Cryo-EM structure of neuropeptide Y Y2 receptor in complex with NPY and Gi
EMD-14666
[28/82]
Cryo-EM structure of Human ACE2 bound to a high-affinity SARS CoV-2 mutant
EMD-32152
[29/82]
Cryo-EM structure of human very long-chain fatty acid ABC transporter ABCD1
EMD-26346
[30/82]
Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
EMD-31926
[32/82]
The overall structure of human chromatin remodeling PBAF-nucleosome complex
EMD-31712
[34/82]
Cryo-EM Structure of Camellia sinensis glutamine synthetase CsGSIb decamer assembly
EMD-24726
[36/82]
afTMEM16 DE/AA mutant in C14 lipid nanodiscs in the presence of Ca2+
EMD-13744
[37/82]
Cryo-EM map of Candida albicans 80S ribosome in complex with phyllanthoside
EMD-24717
[41/82]
afTMEM16 in C22 lipid nanodiscs with MSP2N2 scaffold protein in the presnece of Ca2+
EMD-32401
[42/82]
Cryo-EM structure of N-terminal modified human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs
EMD-25119
[43/82]
Cryo-EM structure of MAP7 MTBD and microtubule-associated protein tau, bound to the microtubule
EMD-32284
[45/82]
Structure of USP14-bound human 26S proteasome in substrate-inhibited state SD5_USP14
EMD-32621
[48/82]
Cryo-EM structure of VWF D'D3 dimer (wild type) complexed with D1D2 at 3.4 angstron resolution (1 unit)
EMD-13967
[49/82]
Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4
EMD-13865
[50/82]
Leishmania major ADP-actin filament decorated with Leishmania major cofilin
EMD-24731
[52/82]
afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, monomer with extra lipids
EMD-32171
[54/82]
Cryo-EM structure of ATP-bound human very long-chain fatty acid ABC transporter ABCD1
EMD-33069
[55/82]
Cryo-EM structure of neuropeptide Y Y1 receptor in complex with NPY and Gi
EMD-32761
[56/82]
Cryo-EM structure of human glucose transporter GLUT4 bound to cytochalasin B in detergent micelles
EMD-33071
[57/82]
Cryo-EM structure of neuropeptide Y Y4 receptor in complex with PP and Gi
EMD-25216
[58/82]
Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 4)
EMD-13977
[59/82]
Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV2) from Corynebacterium glutamicum (as isolated)
EMD-11926
[61/82]
Cryo-EM structure of human exostosin-like 3 (EXTL3) in complex with UDP
EMD-13749
[62/82]
Cryo-EM map of the Candida albicans 80S ribosome in complex with anisomycin
EMD-24730
[64/82]
afTMEM16 in C18 lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+, full dimer
EMD-24727
[67/82]
afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+
EMD-31715
[68/82]
Cryo-EM Structure of Camellia sinensis glutamine synthetase CsGSIb inactive Pentamer State III
EMD-31928
[69/82]
The NBL-HBL module of human chromatin remodeling PBAF-nucleosome complex
EMD-13976
[70/82]
Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV2) from Corynebacterium glutamicum (stigmatellin and azide bound)
EMD-32344
[72/82]
Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex
EMD-25217
[73/82]
Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 3)
EMD-24723
[74/82]
afTMEM16 in C14 lipid nanodiscs with MSP1E3 scaffold protein in the absence of Ca2+
