Tilt pair validation server
Welcome to the PDBe tilt pair validation server!
Tilt-pair validation analysis (Rosenthal
and Henderson, 2003) can be used to assess the accuracy of initial angle assignment in
single-particle processing. To perform this analysis
you need to collect two corresponding sets of particle images - one untilted and the other tilted, then
upload the stacks of images along with a 3D reconstruction
based on the untilted images. This server is based on the Tilt-pair
server developed at MRC National Institute for Medical Research (Wasilewski and Rosenthal, 2014),
and we thank Sebastian Wasilewski and Peter Rosenthal for their help in developing and testing the
current server.
You may upload map files in MRC or CCP4 format, and parameter files (containing Euler angles for
individual particles) in Spider or Frealign format.
We have some test data sets that you can use to try out the service
here. We are still developing the
server and appreciate
your
feedback!
Quick links
Recent Entries
(Show all)Human L-type voltage-gated calcium channel Cav1.2 (CABAD Map 1, Consensus Map)
III2IV2 respiratory supercomplex from Saccharomyces cerevisiae with 4 bound UQ6
Cryo-EM structure of the human Sec61 complex inhibited by cotransin
Cryo-EM structure of native Otu2-bound ubiquitinated 48S initiation complex (partial)
CryoEM structure of the pointy tip (proteins pIII/pVI/pVIII) from the f1 filamentous bacteriophage
Cryo-electron tomogram of an extracellular vesicle containing tau filaments isolated from Alzheimer's Disease patient brain
Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class2
Human L-type voltage-gated calcium channel Cav1.2 complexed with L-leucine (Segment Map 01)
E. coli 50S assembly intermediate from bL17-depletion strain, class: rl17-B-b1
III2IV respiratory supercomplex from Saccharomyces cerevisiae cardiolipin-lacking mutant
28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA
MicroED structure of Proteinase K from lamellae milled from multiple plasma sources
Cryo-EM structure of the human Sec61 complex inhibited by eeyarestatin I
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) in a partially-open apo state (Class 1)
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by cyclotriazadisulfonamide (CADA)
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in intermediate conformer
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in editing conformer (composite)
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by eeyarestatin I
CryoEM reconstruction of tri-specific 298-52-80 Multabody (no symmetry applied)
Paired helical tau filaments from high-spin supernatants of aqueous extracts from Alzheimer's disease brains | PHF Tau
Cryo-EM structure of human TRPV6 in complex with the natural phytoestrogen genistein
Human EMC:human Cav1.2 channel complex in GDN detergent (ECAB Map 2, Consensus Map)
Cryo-EM structure of native Otu2-bound ubiquitinated 43S pre-initiation complex
Yeast cytoplasmic pre-40S ribosome biogenesis complex with deubiquitinating enzyme Otu2
Structure of FFAR1-Gq complex bound to TAK-875 in a lipid nanodisc
Tau Paired Helical Filament from Cellular Fraction of Alzheimer's disease brain
Tau Paired Helical Filament from Extracellular Vesicles from Alzheimer's disease brain (Individual 1)
Human EMC:human Cav1.2 channel complex in GDN detergent (Segment map 02)
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in editing conformer (consensus)
Atomic model of the core modifying region of human fatty acid synthase in complex with TVB-2640 - C2 refinement
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by apratoxin F
Cryo-EM structure of the human Sec61 complex inhibited by ipomoeassin F
Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with L-leucine (CABAD Map 2)
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) in a partially-open apo state (Class 2)
Atomic model of the core modifying region of human fatty acid synthase in complex with TVB-2640
Cryo-EM map 3.8 angstroms of CLOCK-BMAL1 bound to a nuclesomal E-box at position SHL-6.2
CryoEM structure of the round tip (proteins pVII/pVIII/pIX) from the f1 filamentous bacteriophage
SARS-CoV-2 XBB.1 spike glycoprotein in complex with ACE2 (1-up state)
in vitro reconstituted yeast 40S ribosome complex with deubiquitinating enzyme Otu2 bound to ubiquitinated eS7 - body 2 (40S head)
Cryo-EM structure of in vitro reconstituted Otu2-bound Ub-40S complex - body 1
Human mitochondrial DNA polymerase gamma ternary complex with GC basepair
Human EMC:human Cav1.2 channel complex in GDN detergent (ECAB Map 1, Consensus Map)
CryoEM structure of the central filamentous region of the f1 filamentous bacteriophage, consisting of the major capsid protein pVIII
Local refinement map of Otu2 C-terminal domain bound to ubiquitinated eS7 on yeast 40S ribosome
Structure of the TRAP complex with the Sec translocon and a translating ribosome
CryoEM reconstruction of the tri-specific 298-52-80 Multabody (octahedral symmetry applied)
Cryo-EM structure of the human Sec61 complex in a partially-open apo state (Class 1)
E. coli 50S intermediate, bL17-depletion strain, class: rl17-B-b3
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in editing conformer (local refinement of subunit A and primer/template DNA)
E. coli 50S intermediate, bL17-depletion strain, class: rl17-B-b3
Cryo-EM Structure of biliverdin-bound mitochondrial ABC transporter ABCB10 from Biortus
CLOCK-BMAL1 bound to a nucleosome SHL+5.8 (focus refinement with CLOCK-BMAL1 PAS domains masked)
Cryo-EM structure of the human Sec61 complex inhibited by decatransin
Cryo-EM structure of the human Sec61 complex in a partially-open apo state (Class 2)
CLOCK-BMAL1 bound to the native Por enhancer nucleosome at 3.8 angstrom resolution
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by ipomoeassin F (Class 1)
Coagulation factor VIII bound to a patient-derived anti-C1 domain antibody inhibitor
Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2
Cryo-EM Structure of RyR1 + ATP-gamma-S (Local Refinement of TMD)
Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class1
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by cotransin
Local refinement map of Otu2 N-terminal domain bound to yeast 40S ribosome
Human mitochondrial DNA polymerase gamma ternary complex with GT basepair in replication conformer
SARS-CoV-2 XBB.1 spike glycoprotein in complex with ACE2 (2-up state)
Human L-type voltage-gated calcium channel Cav1.2 complexed with L-leucine (Segment Map 02)
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by decatransin
Phospholipase C beta 3 (PLCb3) in complex with Gbg on lipid nanodiscs
Structure of a human EMC:human Cav1.2 channel complex in GDN detergent (ECAB Map 3)
SARS-CoV-2 XBB.1 spike glycoprotein in complex with ACE2 focused on RBD-ACE2 interface
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by mycolactone
Cryo-EM structure of the human Sec61 complex inhibited by mycolactone
Cryo-EM structure of the human Sec61 complex inhibited by cyclotriazadisulfonamide (CADA)
E. coli 50S intermediate, bL17-depletion strain, class: rl17-C-b1
E. coli 50S intermediate, bL17-depletion strain, class: rl17-E-b10
Cryo-EM map of chimeric Sec complex (human Sec61 and yeast Sec63-71-72) inhibited by ipomoeassin F (Class 2)
Atomic model of the core modifying region of human fatty acid synthase
Cryo-EM structure of the human Sec61 complex inhibited by apratoxin F