Tilt pair validation server
Welcome to the PDBe tilt pair validation server!
Tilt-pair validation analysis (Rosenthal
and Henderson, 2003) can be used to assess the accuracy of initial angle assignment in
single-particle processing. To perform this analysis
you need to collect two corresponding sets of particle images - one untilted and the other tilted, then
upload the stacks of images along with a 3D reconstruction
based on the untilted images. This server is based on the Tilt-pair
server developed at MRC National Institute for Medical Research (Wasilewski and Rosenthal, 2014),
and we thank Sebastian Wasilewski and Peter Rosenthal for their help in developing and testing the
current server.
You may upload map files in MRC or CCP4 format, and parameter files (containing Euler angles for
individual particles) in Spider or Frealign format.
We have some test data sets that you can use to try out the service
here. We are still developing the
server and appreciate
your
feedback!
Quick links
Recent Entries
(Show all)Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi1 complex(mask on Gil-scFV16 complex)
Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
Structure of SLC40/ferroportin in complex with vamifeport and synthetic nanobody Sy12 in outward-facing conformation
Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin
Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi
S728-1157 IgG in complex with SARS-CoV-2-6P-Mut7 Spike protein (global refinement)
Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi
Cryo-EM structure of conjugative pili from Pyrobaculum calidifontis
Alpha1/BetaB Heteromeric Glycine Receptor in 1 mM Glycine 20 uM Ivermectin State
Structure of SLC40/ferroportin in complex with synthetic nanobody Sy3 in occluded conformation
Signal peptide mimicry primes Sec61 for client-selective inhibition
Alpha1/BetaB Heteromeric Glycine Receptor in Strychnine-Bound State
Heteromeric ring comprised of peroxiredoxin from Thermococcus kodakaraensis (TkPrx) F42C/C46S/C205S/C211S mutant modified with 2-(bromoacetyl)naphthalene (Naph@TkPrx*F42C) and TkPrx C46S/F76C/C205S/C211S mutant modified with 2-(bromoacetyl)naphthalene (Naph@TkPrx*F76C) (Naph@(MIX|3:3))
Herpes simplex virus 1 polymerase holoenzyme bound to DNA and dTTP in closed conformation
Cryo-EM structure of the TUG891 bound GPR120-Giq complex(mask on Giq-scFV16 complex)
A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom
Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (C1 symmetry)
Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi1 complex(mask on receptor)
Structure of WT E.coli ribosome 50S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site meta-aminobenzoic acid charged NH-tRNAPhe
Cutibacterium acnes 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
Herpes simplex virus 1 polymerase holoenzyme bound to DNA in both open/closed conformations
Acidipropionibacterium acidipropionici encapsulin in a closed state at pH 7.5
Mouse apoferritin from data collected on Talos Arctica microscope equipped with a K3 camera operating in super resolution mode, in 0-50 nm ice thickness
Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in soybean lipids
Human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin (Segment Map)
Structure of SLC40/ferroportin in complex with vamifeport and synthetic nanobody Sy3 in occluded conformation
Herpes simplex virus 1 polymerase holoenzyme bound to DNA and acyclovir triphosphate in closed conformation
Acidipropionibacterium acidipropionici encapsulin in an open state at pH 7.5
Yeast RNA polymerase II transcription pre-initiation complex with core Mediator
Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (D6 symmetry)
Mouse apoferritin from data collected on Talos Arctica microscope equipped with a K3 camera operating in super resolution mode, in 100-150 nm ice thickness
Cryo-EM structure of the RecA presynaptic filament from S.pneumoniae
CryoEM structure of synthetic tau repeat R1R2 with two acetylated lysines at positions 274 and 280
Electron cryo-tomography of the ER-mitochondria encounter structure ERMES
Cryo-EM structure of Mycobacterial Type VII Secretion System Virulence Factor EspB (residues 1-332) with Phosphatidic acid (PA)
Cryo-EM structure of human ABCD1 E630Q in the presence of ATP in inward-facing state
Acidipropionibacterium acidipropionici encapsulin in a closed state at pH 3.0
Mycobacterium tuberculosis RNAP elongation complex with NusG transcription factor
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C6 symmetry)
Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin (consensus map)
Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi complex(consensus map)
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C1 symmetry)
Cryo-EM structure of the TUG891 bound GPR120-Giq complex (consensus map)
Human Cx36/GJD2 gap junction channel with pore-lining N-terminal helices in soybean lipids
Human olfactory receptor OR51E2 bound to propionate in complex with miniGs399
SARS-CoV-2 Delta-RBD complexed with Fabs BA.2-36, BA.2-23, EY6A and COVOX-45
Structural basis for catalysis of human choline/ethanolamine phosphotransferase (CEPT1)
Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
Cryo-EM structure of human ABCD1 E630Q in the presence of ATP and Magnesium in outward-facing state
Cryo-EM structure of Mycobacterial Type VII Secretion System Virulence Factor EspB (residues 1-332)
Mouse apoferritin from data collected on Talos Arctica microscope equipped with a K3 camera operating in super resolution mode, in 150-200 nm ice thickness
Bovine multidrug resistance protein 1 (MRP1) bound to cyclic peptide inhibitor 1 (CPI1)
Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Consensus map)
Octahedral supramolecular assembly of the bicomponent gamma-hemolysin octameric pore complexes from Staphylococcus aureus Newman.
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C1 symmetry)
Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Focused map on PYY(3-36)-Y2R)
The SARS-CoV-2 receptor binding domain bound with the Fab fragment of a human neutralizing antibody Ab712
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C6 symmetry)
Herpes simplex virus 1 polymerase holoenzyme bound to mismatched DNA in editing conformation
Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Focused map on NPY-Y2R)
Structural basis for catalysis of human choline/ethanolamine phosphotransferase (CEPT1) complexed with CDP-choline
S728-1157 IgG in complex with SARS-CoV-2-6P-Mut7 Spike protein (focused refinement)
Structure of dodecameric KaiC-RS-S413E/S414E complexed with KaiB-RS solved by cryo-EM
Mouse apoferritin from data collected on Talos Arctica microscope equipped with a K3 camera operating in super resolution mode, in 50-100 nm ice thickness
A cryo-EM structure of B. oleracea RNA polymerase V at 3.57 Angstrom
Human Oct4 bound to nucleosome with human nMatn1 sequence (focused refinement of Oct4 bound region)
Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains - KWOCA51
The SARS-CoV-2 receptor binding domain bound with the Fab fragment of a human neutralizing antibody Ab847
Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Consensus map)
Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Complete conformation 1 composition)
Cryo-EM structure of the TUG891 bound GPR120-Giq complex(mask on receptor)
Human Oct4 bound to nucleosome with human nMatn1 sequence (focused refinement of nucleosome)
The SARS-CoV-2 receptor binding domain bound with the Fab fragment of a human neutralizing antibody Ab709
Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi (Focused map on Gi-scFv16)
Previously uncharacterized rectangular bacteria in the dolphin mouth
Mycobacterium tuberculosis RNAP paused elongation complex with Escherichia coli NusG transcription factor
Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in detergents at 2.2 Angstroms resolution
human EMC:human Cav1.2 channel complex in GDN detergent at 3.1 Angstrom
Complex structure of Neuropeptide Y Y2 receptor in complex with NPY and Gi (Focused map on Gi-scFv16)
Mouse apoferritin from data collected on Talos Arctica microscope equipped with a K3 camera operating in super resolution mode, in 200-500 nm ice thickness
The SARS-CoV-2 receptor binding domain bound with the Fab fragment of a human neutralizing antibody Ab765
Complex structure of a small molecule (SPC-14) bound SARS-CoV-2 spike protein, closed state