Cryo-EM structure of Saccharomyces cerevisiae TOROID (TORC1 Organized in Inhibited Domains).
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7pqh
Q-score: 0.241
Prouteau M, Bourgoint C, Felix J, Bonadei L, Sadian Y, Gabus C, Savvides SN, Gutsche I, Desfosses A, Loewith R
Nat Struct Mol Biol (2023) [ DOI: doi:10.1038/s41594-022-00912-6 ]
- Target of rapamycin complex 1 subunit kog1,target of rapamycin complex 1 subunit kog1 (183 kDa, Protein from Saccharomyces cerevisiae)
- Torc1 organized in inhibited domains (toroid) (Complex from Saccharomyces cerevisiae)
- Serine/threonine-protein kinase tor2 (281 kDa, Protein from Saccharomyces cerevisiae)
- Target of rapamycin complex subunit lst8 (34 kDa, Protein from Saccharomyces cerevisiae)
Helical reconstruction of TOROID (TORC1 Organized in Inhibited Domains) filaments.
Prouteau M, Bourgoint C, Felix J, Bonadei L, Sadian Y, Gabus C, Savvides SN, Gutsche I, Desfosses A, Loewith R
Nat Struct Mol Biol () [ DOI: doi:10.1038/s41594-022-00912-6 ]
- Serine/threonine-protein kinase tor2 (Protein from Saccharomyces cerevisiae)
- Torc1 organized in inhibited domains (toroid) (Complex from Saccharomyces cerevisiae)
- Target of rapamycin complex 1 subunit kog1 (Protein from Saccharomyces cerevisiae)
- Target of rapamycin complex subunit lst8 (Protein from Saccharomyces cerevisiae)
S. cerevisiae ORC bound to 84 bp ARS1 DNA
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7tjf
Q-score: 0.585
Schmidt JM, Yang R, Kumar A, Hunker O, Seebacher J, Bleichert F
To Be Published
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 4 (61 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Dna, 84 bp ars1 (25 kDa, DNA from Saccharomyces cerevisiae)
- Orc in complex with ars1 dna (Complex)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna, 84 bp ars1 (25 kDa, DNA from Saccharomyces cerevisiae)
Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk
Kulczyk AW, Sorzano COS, Grela P, Tchorzewski M, Tumer NE, Li XP
J Biol Chem (2022) 299 pp. 102795-102795 [ DOI: doi:10.1016/j.jbc.2022.102795 Pubmed: 36528064 ]
Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7u6v
Q-score: 0.201
Kulczyk AW, Sorzano COS, Grela P, Tchorzewski M, Tumer NE, Li XP
J Biol Chem (2022) 299 pp. 102795-102795 [ DOI: doi:10.1016/j.jbc.2022.102795 Pubmed: 36528064 ]
- Native ribosomal p-stalk (Complex from Saccharomyces cerevisiae)
- Shiga toxin 2a (Complex from Shigella dysenteriae)
- C-terminal domain (ctd) from the ribosomal p-stalk (654 Da, Protein from Baker's yeast)
- Shiga toxin 2a subunit a (stx2a) (33 kDa, Protein from Shigella dysenteriae)
- Shiga toxin 2a in complex with the native ribosomal p-stalk (100 kDa, Complex)
- Water (18 Da, Ligand)
- Shiga toxin 2a subunit b (stx2b) (7 kDa, Protein from Shigella dysenteriae)
Enp1TAP-S21_A population of yeast small ribosomal subunit precursors depleted of rpS21/eS21
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8c00
Q-score: 0.535
Poell G, Griesenbeck J, Tschochner H, Milkereit P
To Be Published
- 18s rrna precursor (579 kDa, RNA from baker's yeast)
- 40s ribosomal protein s15 (16 kDa, Protein from baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from baker's yeast)
- Serine/threonine-protein kinase rio2 (49 kDa, Protein from baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from baker's yeast)
- Ribosome biogenesis protein tsr1 (90 kDa, Protein from baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from baker's yeast)
- Rps5 (25 kDa, Protein from baker's yeast)
- Enp1-tap associated immature ribosomal particles from s. cerevisiae depleted of rps21/es21 (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from baker's yeast)
- 40s ribosomal protein s18-a (17 kDa, Protein from baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from baker's yeast)
Enp1TAP_A, consensus refinement
Poell G, Griesenbeck J, Tschochner H, Milkereit P
To Be Published
Enp1TAP_A population of yeast small ribosomal subunit precursors, multibody refinement
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8c01
Q-score: 0.569
Poell G, Griesenbeck J, Tschochner H, Milkereit P
To Be Published
- 40s ribosomal protein s15 (16 kDa, Protein from baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from baker's yeast)
- Serine/threonine-protein kinase rio2 (49 kDa, Protein from baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from baker's yeast)
- Ribosome biogenesis protein tsr1 (90 kDa, Protein from baker's yeast)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s13 (17 kDa, Protein from baker's yeast)
- Enp1-tap associated immature ribosomal particles from s. cerevisiae (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from baker's yeast)
- 40s ribosomal protein s17-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from baker's yeast)
- 40s ribosomal protein s18-a (17 kDa, Protein from baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from baker's yeast)
- 40s ribosomal protein s0-a (28 kDa, Protein from baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from baker's yeast)
- 18s rrna precursor (581 kDa, RNA from baker's yeast)
- 20s-pre-rrna d-site endonuclease nob1 (51 kDa, Protein from baker's yeast)
- 40s ribosomal protein s21-a (9 kDa, Protein from baker's yeast)
- 40s ribosomal protein s2 (27 kDa, Protein from baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from baker's yeast)
Enp1TAP_B, multibody refinement
Poell G, Griesenbeck J, Tschochner H, Milkereit P
To Be Published