Benchmark SBF SEM data of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy
Krentzel D, Elphick M, Domart M, Peddie CJ, Laine RF, Henriques R, Collinson LM, Jones ML
bioRxiv (2023) [ DOI: 10.1101/2023.05.11.540445 ]
Image sets:
- High magnification aligned full resolution SBF SEM image stack
- Low magnification raw SBF SEM overview images (ROI00) of the region around the cell imaged in ROI01
- High magnification aligned downscaled to 28nm XY SBF SEM image stack
- Low magnification raw SBF SEM overview images (ROI00) of HeLa cell previously imaged by Airyscan
- High magnification aligned downscaled to 14nm XY SBF SEM image stack
Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy
Krentzel D, Elphick M, Domart M, Peddie CJ, Laine RF, Henriques R, Collinson LM, Jones ML
bioRxiv (2023) [ DOI: 10.1101/2023.05.11.540445 ]
Image sets:
- Aligned full resolution FIB SEM stack resliced to match the Airyscan dataset
- Run 3
- Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset
- Run 2
- Raw FIB SEM Run 1
Choi ML, Chappard A, Singh BP, Maclachlan C, Rodrigues M, Fedotova E, Berezhnov AV, De S, Peddie C, Athauda D, Virdi GS, Zhang W, Evans JR, Wernick A, Zanjani ZS, Angelova PR, Esteras N, Vinikurov A, Morris K, Jeacock K, Tosatto L, Little D, Gissen P, Clarke DJ, Kunath T, Collinson L, Klenerman D, Abramov AY, Horrocks MH, Gandhi S
bioRxiv (2022) [ DOI: 10.1101/2022.06.07.494932 ]
Image sets:
- FIB-SEM data set of a neuronal cell in culture (Dish 51)
- Unaligned serial TEM imaging data of a neuronal cell in culture (dish42)
- Unaligned Serial TEM Imaging data of a neuronal cell in culture (Dish 43)
- FIB-SEM data of a neuronal cell in culture (Dish 52)
- FIB-SEM data of a neuronal cell in culture (Dish 141)
- FIB-SEM data of a neuronal cell in culture (Dish 131)
- Unaligned TEM serial imaging data of a neuronal cell in culture (dish41)
Light and electron microscopy continuum-resolution imaging of 3D cell cultures
DImprima EDI, Garcia Montero MGM, Gawrzak, SG, Ronchi PR, Zagoriy IZ, Schwab YS, Jechlinger JS, Mahamid JM
Dev. Cell ()
Image sets:
Processed FIB-SEM images of murine hypothalamus-derived GT1-7 neuronal cells
Lumkwana D, Peddie CJ, Kriel J, Michie LL, Heathcote N, Collinson LM, Kinnear C, Loos B
Frontiers in cell and developmental biology (2022) [ DOI: 10.3389/fcell.2022.819571 ]
Image sets:
- MRC013_EM04295_04_8O_XZ: 1 micromolar spermidine / bafilomycin treatment
- MRC013_EM04295_01_7J_XZ: Control treatment
- MRC013_EM04295_06_6K_XZ: 10 micromolar spermidine / bafilomycin treatment
- MRC013_EM04295_05_8Q_XZ: 10 micromolar spermidine treatment
- MRC013_EM04295_02_7M_XZ: Bafilomycin treatment
- MRC013_EM04295_03_9M_XZ: 1 micromolar spermidine treatment
Benchmark FIB SEM data of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy
Krentzel D, Laine RF, Domart MC, Peddie CJ, Henriques R, Collinson LM, Jones ML
To Be Published
Image sets:
- Aligned full resolution FIB SEM stack in its original orientation
- Raw FIB SEM run 2
- Raw FIB SEM run 1 part 2
- Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset
- Aligned full resolution FIB SEM stack resliced to match the orientation of the Airyscan data
- Downscaled (10nm) aligned FIB SEM stack resliced to match the Airyscan dataset
- Raw FIB SEM run 1 part 3
- Raw FIB SEM run 1 part 1
Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S
Dis Model Mech (2020) 13 [ DOI: 10.1242/dmm.043091 Pubmed: 32461265 ]
Image sets:
- Survey SBF SEM images showing the position of the imaged ROIs at the start of each run (3 runs- #20-22)
- High magnification raw SBF SEM images (ROI02) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain (run 20)
- Low magnification raw SBF SEM overview images (ROI00) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain over 3 runs (20-22)
- High magnification raw SBF SEM images (ROI01) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain over 3 runs (20-22)
- High magnification raw SBF SEM images (ROI02) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain for runs 21-22
SBF SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites
Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S
Dis Model Mech (2020) 13 [ DOI: 10.1242/dmm.043091 Pubmed: 32461265 ]
Image sets:
- High magnification raw SBF SEM images (ROI01) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage
- Low magnification raw SBF SEM overview images (ROI00) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage
- Survey SBF SEM image showing the position of the imaged ROIs at the start of the run
TEM images of a Zebrafish hindbrain cells containing Toxoplasma gondii tachizoites
Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S
Dis Model Mech (2020) 13 [ DOI: 10.1242/dmm.043091 Pubmed: 32461265 ]
Image sets:
- Non aligned TEM image stack of a zebrafish hindbrain cell containing a Toxoplasma Gondii tachizoite (toxo 2)
- Non aligned TEM image stack of a zebrafish hindbrain cell containing a Toxoplasma Gondii tachizoite (toxo 3)
- Non aligned TEM image stack of a zebrafish hindbrain cell containing a Toxoplasma Gondii tachizoite (toxo 1)
Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S
Dis Model Mech (2020) 13 [ DOI: 10.1242/dmm.043091 Pubmed: 32461265 ]
Image sets:
- Survey SBF SEM image showing the position of the 3 imaged ROIs at the start of the run
- Low magnification raw SBF SEM overview images (ROI00) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage
- High magnification raw SBF SEM images (ROI01) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage