Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8fd7
Q-score: 0.406
To Be Published
- Voltage-dependent l-type calcium channel subunit beta-3 (53 kDa, Protein from Oryctolagus cuniculus)
- Rabbit cav beta3 (100 kDa, Complex from Oryctolagus cuniculus)
- Water (18 Da, Ligand)
- Ternary complex of human cav alpha1c with rabbit cav alpha2delta-1 and rabbit cav beta3 (Cell from Homo sapiens)
- (2r)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl heptadecanoate (649 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Voltage-dependent l-type calcium channel subunit alpha-1c (187 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- [1-(aminomethyl)cyclohexyl]acetic acid (171 Da, Ligand)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl dodecanoate (579 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit cav alpha2delta-1 (125 kDa, Complex from Oryctolagus cuniculus)
- Human cav alpha1c (187 kDa, Complex from Homo sapiens)
- Voltage-dependent calcium channel subunit alpha-2/delta-1 (125 kDa, Protein from Oryctolagus cuniculus)
human EMC:human Cav1.2 channel complex in GDN detergent at 3.1 Angstrom
To Be Published
- Ternary complex of the human er membrane protein complex (emc) with human cav alpha1c and rabbit cav beta3 (Complex from Homo sapiens)
- Rabbit cav beta3 (100 kDa, Complex from Oryctolagus cuniculus)
- Er membrane protein complex subunit 1 (112 kDa, Complex from Homo sapiens)
- Human cav alpha1c (187 kDa, Complex from Homo sapiens)
To Be Published
- Rabbit cav beta3 (100 kDa, Complex from Oryctolagus cuniculus)
- Rabbit cav alpha2delta-1 (125 kDa, Complex from Oryctolagus cuniculus)
- Human cav alpha1c (187 kDa, Complex from Homo sapiens)
- Ternary complex of human cav alpha1c with rabbit cav alpha2delta-1 and rabbit cav beta3 (Cell from Homo sapiens)
CryoEM structure of SPCA1a in E1-Ca-AMPPCP state subclass 1
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
Sci Adv (2023) 9 pp. eadd9742-eadd9742 [ DOI: doi:10.1126/sciadv.add9742 Pubmed: 36867705 ]
- Calcium-transporting atpase type 2c member 1 (103 kDa, Protein from Homo sapiens)
- Spca1a (Complex from Homo sapiens)
- A complex of spca1a and megabody in e1-ca-amppcp state, subclass1 (Complex)
- Calcium ion (40 Da, Ligand)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Nanobody head piece of megabody (13 kDa, Protein from Vicugna pacos)
- Megabody (Complex from Vicugna pacos)
CryoEM structure of SPCA1a in E1-Ca-AMPPCP state subclass 2
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
Sci Adv (2023) 9 pp. eadd9742-eadd9742 [ DOI: doi:10.1126/sciadv.add9742 Pubmed: 36867705 ]
- A complex of spca1a and megabody in e1-ca-amppcp state, subclass2 (Complex)
- Calcium-transporting atpase type 2c member 1 (103 kDa, Protein from Homo sapiens)
- Spca1a (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Megabody (Complex from Vicugna pacos)
- The nanobody head piece of megabody. (13 kDa, Protein from Vicugna pacos)
CryoEM structure of SPCA1a in E1-Ca-AMPPCP state subclass 3
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
Sci Adv (2023) 9 pp. eadd9742-eadd9742 [ DOI: doi:10.1126/sciadv.add9742 Pubmed: 36867705 ]
- Nanobody head piece of megabody (13 kDa, Protein from Vicugna pacos)
- A complex of spca1a and megabody in e1-ca-amppcp state, subclass3 (Complex)
- Calcium-transporting atpase type 2c member 1 (103 kDa, Protein from Homo sapiens)
- Spca1a (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Megabody (Complex from Vicugna pacos)
CryoEM structure of SPCA1a in E1-Mn-AMPPCP state subclass 1
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
Sci Adv (2023) 9 pp. eadd9742-eadd9742 [ DOI: doi:10.1126/sciadv.add9742 Pubmed: 36867705 ]
- Nanobody head piece of megabody (13 kDa, Protein from Vicugna pacos)
- Calcium-transporting atpase type 2c member 1 (103 kDa, Protein from Homo sapiens)
- Spca1a (Complex from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
- A complex of spca1a and megabody in e1-mn-amppcp state, subclass1 (Complex)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Megabody (Complex from Vicugna pacos)
CryoEM structure of SPCA1a in E2P state
Chen Z, Watanabe S, Hashida H, Inoue M, Daigaku Y, Kikkawa M, Inaba K
Sci Adv (2023) 9 pp. eadd9742-eadd9742 [ DOI: doi:10.1126/sciadv.add9742 Pubmed: 36867705 ]
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Calcium-transporting atpase type 2c member 1 (103 kDa, Protein from Homo sapiens)
- Spca1a in e2p state (Complex from Homo sapiens)
The cryo-EM structure of human pre-17S U2 snRNP
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8hk1
Q-score: 0.317
Yang F, Bian T, Zhan X, Chen Z, Xing Z, Larsen NA, Zhang X, Shi Y
Nat Commun (2023) 14 pp. 897-897 [ Pubmed: 36797247 DOI: doi:10.1038/s41467-023-36489-x ]
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- U2 snrna (60 kDa, RNA from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- [(2~{s},3~{s},4~{e},6~{s},7~{r},10~{r})-3,7-dimethyl-2-[(2~{e},4~{e},6~{r})-6-methyl-6-oxidanyl-7-[(2~{r},3~{r})-3-[(2~{r},3~{s})-3-oxidanylpentan-2-yl]oxiran-2-yl]hepta-2,4-dien-2-yl]-7,10-bis(oxidanyl)-12-oxidanylidene-1-oxacyclododec-4-en-6-yl] 4-cycloheptylpiperazine-1-carboxylate (718 Da, Ligand)
- Splicing factor 3a subunit 1 (88 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Hiv tat-specific factor 1 (85 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- The human pre-17s u2 snrnp (Complex from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Atp-dependent rna helicase ddx42 (103 kDa, Protein from human)