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grant_code:"RR140082"

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Current Database

  • EMDB [23]

Current status

  • REL [23]

Sample type

  • Cellular component [17]
  • Cell [6]
  • Ligand [1]
  • Protein [1]

Organism

  • Mus musculus [10]
  • Saccharomyces cerevisiae [6]
  • Chlamydomonas reinhardtii [4]
  • Salpingoeca rosetta (strain atcc 50818 / bsb-021) [3]

EM Method

  • Tomography [10]
  • Subtomogram averaging [9]
  • Single-particle [4]

Resolution

  • ≥ 4Å and < 5Å [2]
  • ≥ 6Å and < 8Å [2]
  • ≥ 16Å and < 20Å [1]
  • ≥ 20Å and < 30Å [8]
  • ≥ 30Å [3]

Model molecular weight

  • 2-5 MDa [1]

Release Date

  • 2025 [7]
  • 2022 [12]
  • 2021 [4]

Author by name

  • Nicastro D [23]
  • Zhao Y [14]
  • Gui L [13]
  • Reetz E [9]
  • Daniela N [7]
  • Dzeja PP [7]
  • Fernandez-gonzalez A [7]
  • Mitsialis SA [7]
  • Song K [7]
  • Tavakoli A [7]
  • Xuewu Z [7]
  • Yanhe Z [7]
  • Zhang S [7]
  • Zhang X [7]
  • Henne WM [6]
  • Kovalenko A [6]
  • Rogers S [6]
  • Cai K [4]
  • Cheng X [4]
  • Hou Y [4]
  • Phan N [4]
  • Witman GB [4]
  • Zhao L [4]
  • Chung JJ [3]
  • Davies KM [3]
  • Huang X [3]
  • Hwang J [3]
  • Hwang JY [3]
  • Lishko PV [3]
  • Pinskey JM [3]
  • Shah NB [3]
  • Wang H [3]
  • Wang TE [3]
  • Wennemuth G [3]
  • Wiesehoefer C [3]

Author by ORCID

  • Nicastro D [15]
  • Mitsialis SA [7]
  • Gui L [7]
  • Zhang X [7]
  • Tavakoli A [7]
  • Zhang S [7]
  • Zhao Y [4]
  • Cai K [4]
  • Witman GB [4]
  • Phan N [4]
  • Cheng X [4]
  • Chung JJ [3]
  • Davies KM [3]
  • Hwang JY [3]
  • Wiesehoefer C [3]
  • Wang H [3]
  • Lishko PV [3]
  • Wennemuth G [3]
  • Pinskey JM [1]

Journal

  • NAT STRUCT MOL BIOL [7]
  • TO BE PUBLISHED [6]
  • J CELL SCI [4]
  • ELIFE [3]
  • NAT COMMUN [3]

Software

  • CRYOSPARC [4]
  • ETOMO [4]
  • PEET [3]
  • RELION [3]
  • 3DMOD [1]

Microscope

  • FEI TITAN KRIOS [20]
  • FEI TECNAI F30 [3]

Electron Source

  • FIELD EMISSION GUN [23]

Film or detector model

  • GATAN K3 BIOQUANTUM 6K X 4K [10]
  • GATAN K3 6K X 4K [9]
  • GATAN ULTRASCAN 1000 2K X 2K [3]
  • GATAN K2 SUMMIT 4K X 4K [1]

External links

  • AlphaFold DB [1]
  • ChEBI [1]
  • ChEMBL [1]
  • DrugBank [1]
  • Gene Ontology [1]
  • InterPro [1]
  • PDB [1]
  • PDBe-Kb [1]
  • Pfam [1]
  • UniProt [1]
Sort by: Download
Showing 1 - 10 of 23. Page 1 of 3
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EMD-46484
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3D View
Cryo-electron tomography and subtomogram averaging of WT mouse respiratory ciliary axoneme
CryoEM
Release date: Aug. 13, 2025
Resolution: 36.0 Å
EM Method: Subtomogram averaging

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46485
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3D View
Cryo-electron tomography and subtomogram averaging of AK1-/- mouse respiratory ciliary axoneme
CryoEM
Release date: Aug. 13, 2025
Resolution: 44.0 Å
EM Method: Subtomogram averaging

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46486
Download Map
3D View
Cryo-electron tomography and subtomogram averaging of AK7-/- mouse respiratory ciliary axoneme
CryoEM
Release date: Aug. 13, 2025
Resolution: 38.0 Å
EM Method: Subtomogram averaging

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46490
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3D View
The cryo-EM structure of RS3 of mouse respiratory cilia
Consensus map
CryoEM
Release date: Aug. 13, 2025
Resolution: 6.5 Å
EM Method: Single-particle

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46491
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3D View
The cryo-EM structure of RS3 head and neck of mouse respiratory cilia
Focused map
CryoEM
Release date: Aug. 13, 2025
Resolution: 7.1 Å
EM Method: Single-particle

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46492
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3D View
The cryo-EM structure of RS3 base and stalk of mouse respiratory cilia
Focused map
CryoEM
Release date: Aug. 13, 2025
Resolution: 4.7 Å
EM Method: Single-particle

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-46494
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3D View
3D structure and atomic structure of RS3 of mouse respiratory cilia
Composite map
CryoEM
Release date: Aug. 13, 2025
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 9d2f
Q-score: 0.23
Similar entry percentile: 34%

Zhao Y, Song K, Tavakoli A, Gui L, Fernandez-Gonzalez A, Zhang S, Dzeja PP, Mitsialis SA, Zhang X, Nicastro D

Nat Struct Mol Biol (2025) 32 pp. 1542-1554 [ DOI: doi:10.1038/s41594-025-01594-6 Pubmed: 40579595 ]

EMD-26204
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3D View
Ciliary 96-nm repeat unit from Salpingoeca rosetta choanoflagellate , generated via cryo-electron tomography and subtomogram averaging
CryoEM
Release date: Nov. 23, 2022
Resolution: 22.0 Å
EM Method: Tomography

Pinskey JM, Nicastro D

eLife (2022) [ ]

EMD-26209
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3D View
Subtomogram average of central pair complex from Salpingoeca rosetta choanoflagellate
CryoEM
Release date: Nov. 23, 2022
Resolution: 25.0 Å
EM Method: Tomography

Pinskey JM, Nicastro D

eLife (2022) [ ]

EMD-26210
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3D View
Barb-like structure on the external surface of the Salpingoeca rosetta choanoflagellate ciliary membrane
CryoEM
Release date: Nov. 23, 2022
EM Method: Tomography

Pinskey JM, Nicastro D

eLife (2022) [ ]

Sort by: Download
Showing 1 - 10 of 23. Page 1 of 3
Display:
Statistics
×

Download


Preview 10 entries View All Download file

Filter By

Current Database

  • EMDB [23]

Current status

  • REL [23]

Sample type

  • Cellular component [17]
  • Cell [6]
  • Ligand [1]
  • Protein [1]

Organism

  • Mus musculus [10]
  • Saccharomyces cerevisiae [6]
  • Chlamydomonas reinhardtii [4]
  • Salpingoeca rosetta (strain atcc 50818 / bsb-021) [3]

EM Method

  • Tomography [10]
  • Subtomogram averaging [9]
  • Single-particle [4]

Resolution

  • ≥ 4Å and < 5Å [2]
  • ≥ 6Å and < 8Å [2]
  • ≥ 16Å and < 20Å [1]
  • ≥ 20Å and < 30Å [8]
  • ≥ 30Å [3]

Model molecular weight

  • 2-5 MDa [1]

Release Date

  • 2025 [7]
  • 2022 [12]
  • 2021 [4]

Author by name

  • Nicastro D [23]
  • Zhao Y [14]
  • Gui L [13]
  • Reetz E [9]
  • Daniela N [7]
  • Dzeja PP [7]
  • Fernandez-gonzalez A [7]
  • Mitsialis SA [7]
  • Song K [7]
  • Tavakoli A [7]
  • Xuewu Z [7]
  • Yanhe Z [7]
  • Zhang S [7]
  • Zhang X [7]
  • Henne WM [6]
  • Kovalenko A [6]
  • Rogers S [6]
  • Cai K [4]
  • Cheng X [4]
  • Hou Y [4]
  • Phan N [4]
  • Witman GB [4]
  • Zhao L [4]
  • Chung JJ [3]
  • Davies KM [3]
  • Huang X [3]
  • Hwang J [3]
  • Hwang JY [3]
  • Lishko PV [3]
  • Pinskey JM [3]
  • Shah NB [3]
  • Wang H [3]
  • Wang TE [3]
  • Wennemuth G [3]
  • Wiesehoefer C [3]

Author by ORCID

  • Nicastro D [15]
  • Mitsialis SA [7]
  • Gui L [7]
  • Zhang X [7]
  • Tavakoli A [7]
  • Zhang S [7]
  • Zhao Y [4]
  • Cai K [4]
  • Witman GB [4]
  • Phan N [4]
  • Cheng X [4]
  • Chung JJ [3]
  • Davies KM [3]
  • Hwang JY [3]
  • Wiesehoefer C [3]
  • Wang H [3]
  • Lishko PV [3]
  • Wennemuth G [3]
  • Pinskey JM [1]

Journal

  • NAT STRUCT MOL BIOL [7]
  • TO BE PUBLISHED [6]
  • J CELL SCI [4]
  • ELIFE [3]
  • NAT COMMUN [3]

Software

  • CRYOSPARC [4]
  • ETOMO [4]
  • PEET [3]
  • RELION [3]
  • 3DMOD [1]

Microscope

  • FEI TITAN KRIOS [20]
  • FEI TECNAI F30 [3]

Electron Source

  • FIELD EMISSION GUN [23]

Film or detector model

  • GATAN K3 BIOQUANTUM 6K X 4K [10]
  • GATAN K3 6K X 4K [9]
  • GATAN ULTRASCAN 1000 2K X 2K [3]
  • GATAN K2 SUMMIT 4K X 4K [1]

External links

  • AlphaFold DB [1]
  • ChEBI [1]
  • ChEMBL [1]
  • DrugBank [1]
  • Gene Ontology [1]
  • InterPro [1]
  • PDB [1]
  • PDBe-Kb [1]
  • Pfam [1]
  • UniProt [1]