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author:"Onishi K"

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Current Database

  • EMDB [9]

Current status

  • REL [9]

Sample type

  • Complex [9]
  • DNA [9]
  • Protein [9]
  • RNA [9]
  • Ligand [6]

Organism

  • Staphylococcus aureus [6]
  • Lachnospiraceae bacterium nd2006 [3]
  • Bacterium [2]
  • Thermanaerosceptrum fracticalcis [1]
  • Dna molecule [1]

EM Method

  • Single-particle [9]

Resolution

  • ≥ 2Å and < 3Å [6]
  • ≥ 3Å and < 4Å [3]

Release Date

  • 2026 [5]
  • 2025 [4]

Author by name

  • Nureki O [9]
  • Omura SN [9]
  • Onishi K [9]
  • Hiramoto T [6]
  • Hirano H [6]
  • Horinaka K [6]
  • Ishiguro S [6]
  • Jividen K [6]
  • Kajimoto S [6]
  • Kashiwakura Y [6]
  • Mori H [6]
  • Nakagawa R [6]
  • Nishimasu H [6]
  • Ohmori T [6]
  • Okazaki S [6]
  • Tanaka M [6]
  • Tsai SQ [6]
  • Yachie N [6]
  • Yamashita K [6]
  • Alfonse L [3]
  • Ditommaso T [3]
  • Garrity AJ [3]
  • Kobayashi TA [3]
  • Koonin EV [3]
  • Maben Z [3]
  • Makarova KS [3]
  • Morinaga H [3]
  • Munoz G [3]
  • Ornstein A [3]
  • Shmakov SA [3]

Journal

  • NAT COMMUN [6]
  • TO BE PUBLISHED [3]

Software

  • CRYOSPARC [3]

Microscope

  • TFS KRIOS [9]

Electron Source

  • FIELD EMISSION GUN [9]

Film or detector model

  • GATAN K3 6K X 4K [6]
  • GATAN K3 BIOQUANTUM 6K X 4K [3]

External links

  • AlphaFold DB [7]
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EMD-69297
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Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the TS-cleaving state
CryoEM
Release date: Sept. 2, 2026
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 23vu
Q-score: 0.623
Similar entry percentile: 91%

Morinaga H, Omura SN, Alfonse L, Ornstein A, Kobayashi TA, Onishi K, Makarova KS, Shmakov SA, Munoz G, Garrity AJ, DiTommaso T, Koonin EV, Maben Z, Nureki O

To Be Published

EMD-69298
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3D View
Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the NTS-cleaving state
CryoEM
Release date: Sept. 2, 2026
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 23vv
Q-score: 0.581
Similar entry percentile: 86%

Morinaga H, Omura SN, Alfonse L, Ornstein A, Kobayashi TA, Onishi K, Makarova KS, Shmakov SA, Munoz G, Garrity AJ, DiTommaso T, Koonin EV, Maben Z, Nureki O

To Be Published

EMD-69299
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3D View
Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex
CryoEM
Release date: Sept. 2, 2026
Resolution: 2.39 Å
EM Method: Single-particle
Fitted PDBs: 23vw
Q-score: 0.629
Similar entry percentile: 80%

Morinaga H, Omura SN, Alfonse L, Ornstein A, Kobayashi TA, Onishi K, Makarova KS, Shmakov SA, Munoz G, Garrity AJ, DiTommaso T, Koonin EV, Maben Z, Nureki O

To Be Published

EMD-63767
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Cryo-EM structure of wild-type SaCas9-guide RNA-mismatched target DNA complex
CryoEM
Release date: March 18, 2026
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 9mb6

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

EMD-63768
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Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex
CryoEM
Release date: March 18, 2026
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9mb7

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

EMD-39941
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Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an interrogation state
CryoEM
Release date: Nov. 5, 2025
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8zcy
Q-score: 0.342
Similar entry percentile: 8%

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

EMD-39942
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3D View
Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an intermediate state
CryoEM
Release date: Nov. 5, 2025
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8zcz
Q-score: 0.484
Similar entry percentile: 41%

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

EMD-39944
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Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in a translocation state
CryoEM
Release date: Nov. 5, 2025
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8zd0
Q-score: 0.572
Similar entry percentile: 78%

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

EMD-39954
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Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in a catalytically active state
CryoEM
Release date: Nov. 5, 2025
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8zda
Q-score: 0.478
Similar entry percentile: 50%

Omura SN, Nakagawa R, Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K, Tsai SQ, Yachie N, Ohmori T, Nishimasu H, Nureki O

Nat Commun (2026) 17 [ Pubmed: 41991526 DOI: doi:10.1038/s41467-026-71626-2 ]

Sort by: Download
Showing 1 - 9 of 9. Page 1 of 1
Display:
Statistics
×

Download


Preview 10 entries View All Download file

Filter By

Current Database

  • EMDB [9]

Current status

  • REL [9]

Sample type

  • Complex [9]
  • DNA [9]
  • Protein [9]
  • RNA [9]
  • Ligand [6]

Organism

  • Staphylococcus aureus [6]
  • Lachnospiraceae bacterium nd2006 [3]
  • Bacterium [2]
  • Thermanaerosceptrum fracticalcis [1]
  • Dna molecule [1]

EM Method

  • Single-particle [9]

Resolution

  • ≥ 2Å and < 3Å [6]
  • ≥ 3Å and < 4Å [3]

Release Date

  • 2026 [5]
  • 2025 [4]

Author by name

  • Nureki O [9]
  • Omura SN [9]
  • Onishi K [9]
  • Hiramoto T [6]
  • Hirano H [6]
  • Horinaka K [6]
  • Ishiguro S [6]
  • Jividen K [6]
  • Kajimoto S [6]
  • Kashiwakura Y [6]
  • Mori H [6]
  • Nakagawa R [6]
  • Nishimasu H [6]
  • Ohmori T [6]
  • Okazaki S [6]
  • Tanaka M [6]
  • Tsai SQ [6]
  • Yachie N [6]
  • Yamashita K [6]
  • Alfonse L [3]
  • Ditommaso T [3]
  • Garrity AJ [3]
  • Kobayashi TA [3]
  • Koonin EV [3]
  • Maben Z [3]
  • Makarova KS [3]
  • Morinaga H [3]
  • Munoz G [3]
  • Ornstein A [3]
  • Shmakov SA [3]

Journal

  • NAT COMMUN [6]
  • TO BE PUBLISHED [3]

Software

  • CRYOSPARC [3]

Microscope

  • TFS KRIOS [9]

Electron Source

  • FIELD EMISSION GUN [9]

Film or detector model

  • GATAN K3 6K X 4K [6]
  • GATAN K3 BIOQUANTUM 6K X 4K [3]

External links

  • AlphaFold DB [7]