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Digitized on PDS 1010M densitometer. Other relevent references K. A. Taylor, M. C. Reedy, L. Cordova and M. K. Reedy. 3-D structure of insect flight muscle in rigor from tilted thin sections. Nature 310, 28 5-291 (1984). Hanspeter Winkler and Kenneth A. Taylor. Multivariate statistical analysis of three-dimensional cross-bridge motifs in insect flight muscle. Ultramicroscopy 77, 141-152 (1999). Chen, Li Fan, Winkler, Hanspeter, Reedy, Michael K., Ree dy, Mary C. and Taylor, Kenneth A.. Molecular Modeling of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle. J. Struct. Biol. 138(2), 92-104 (2002)", "final_reconstruction": { "algorithm": "OTHER", "details": "The reconstruction was done by aligning the members of\n the tilt series using crosscorrelation functions, such as\n phase only and the VanHeel Mutual Correlation Function.\n The sampling in each micrographof a tilted specimen was\n match by interpolation to th e lower angle members of the\n tilt series. This matching of areas and sampling was done\n by a 4 parameter grid search. Once aligned and scaled, the\n data were combined in Fourier space using a\n Whittaker-Shannon interpolation scheme. The map was calculated\n using a reverse Fourier transformation. The software used for\n the reconstruction was in-house and adapted from in some\n cases from MRC software.", "number_images_used": 30, "resolution": { "res_type": "BY AUTHOR", "units": "\u212b", "valueOf_": "40.0" }, "resolution_method": "OTHER" }, "image_processing_id": 1, "instance_type": "tomography_processing" } ], "instance_type": "structure_determination", "method": "tomography", "microscopy_list": { "microscopy": [ { "acceleration_voltage": { "units": "kV", "valueOf_": "100" }, "electron_source": "TUNGSTEN HAIRPIN", "illumination_mode": "FLOOD BEAM", "image_recording_list": { "image_recording": [ { "bits_per_pixel": 16.0, "digitization_details": { "sampling_interval": { "units": "\u00b5m", "valueOf_": "25" }, "scanner": "PERKIN ELMER" }, "instance_type": "image_recording", "number_real_images": 24 } ] }, "imaging_mode": "BRIGHT FIELD", "instance_type": "tomography_microscopy", "microscope": "FEI/PHILIPS EM400", "microscopy_id": 1, "nominal_magnification": 17000.0, "specimen_holder": "Philips rotation-tilt holder", "specimen_holder_model": "PHILIPS ROTATION HOLDER", "temperature": { "temperature_average": { "units": "K", "valueOf_": "25" }, "temperature_max": { "units": "K", "valueOf_": "25" }, "temperature_min": { "units": "K", "valueOf_": "25" } }, "tilt_series": [ { "axis1": { "angle_increment": { "units": "deg", "valueOf_": "10" }, "max_angle": { "units": "deg", "valueOf_": "59.4" }, "min_angle": { "units": "deg", "valueOf_": "4.3" } }, "instance_type": "tilt_series" } ] } ] }, "specimen_preparation_list": { "specimen_preparation": [ { "instance_type": "tomography_preparation", "preparation_id": 1, "vitrification": { "cryogen_name": "NONE" } } ] }, "structure_determination_id": 1 } ] }, "version": "3.0.1.1" }