EMD-12893

Single-particle
3.0 Å
EMD-12893 Deposition: 09/05/2021
Map released: 09/06/2021
Last modified: 09/06/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-12893

Drs2p-Cdc50p in the E1 state

EMD-12893

Single-particle
3.0 Å
EMD-12893 Deposition: 09/05/2021
Map released: 09/06/2021
Last modified: 09/06/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.6 mg/mL
Details: Purified in detergent lauryl maltose neopentyl glycol (LMNG)
Buffer
pH: 7.0
Buffer components [5]:
Name Formula Concentration ChEBI
- MOPS-Tris 50.0 mM
- KCl 100.0 mM
- MgCl2 5.0 mM
Dithiothreitol DTT 1.0 mM
Lauryl maltose neopentyl glycol LMNG 0.03 mg/mL
Grid
Mesh: 400
Model: C-flat-1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY ARRAY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277.15 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Specialist optics
Energy filter
Name: GIF Bioquantum
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K3 (6k x 4k)
Number of grids: 1
Number of real images: 2074
Average exposure time: 1.5 s
Average electron dose per image: 60.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 167226
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CryoSPARC 2 Non-uniform refinement
Startup model [1]
Type: OTHER
Details:Ab initio determined in cryoSPARC v2
Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 Heterogeneous refinement
Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2 Non-uniform refinement
Particle selection [1]
Selected Ref. model Method Software Details
780933 - - - -
Final 3D classification
Software [1]
Name Version Details
CryoSPARC 2 Heterogeneous refinement
CTF correction
Software [1]
Name Version Details
CryoSPARC 2 Patch CTF
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Sharpened map
Geometry
X Y Z
Dimensions 256 256 256
Origin 0 0 0
Spacing 256 256 256
Voxel size 1.0312 Å 1.0312 Å 1.0312 Å
Contour list
Primary Level Source
True 0.185 AUTHOR