Uploading files: FTP & Aspera

Once your submission files have been prepared using the EgaCryptor, the resulting encrypted files and associated md5sum files can be uploaded to your submission account using Aspera or FTP.  

 

Using Aspera to upload your prepared files

Downloading Aspera

Using Aspera

 

Downloading the Aspera ascp command line program

Aspera is a commercial file transfer protocol that may provide faster transfer speeds than ftp especially over longer distances.

Operating System: Windows XP / 2003 / Vista / 2008 / 7 / 8, Mac OS Intel 10.5 / 10.6 / 10.7 / 10.8 / Linux

 

The Aspera ascp command line client can be downloaded here.

Please select *Aspera Connect*.

The ascp command line client is distributed as part of the aspera connect high-performance transfer browser plug-in and is free to use, without registration. 

 

The location of the 'ascp' program in the filesystem:

Mac: on the desktop go cd /Applications/Aspera\ Connect.app/Contents/Resources/ there you'll see the command line utilities where you're going to use 'ascp'.

Windows: the downloaded files are a bit hidden. For instance in Windows 7 the ascp.exe is located in the users home directory in: AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe

Linux: should be in your user's home directory, cd /home/username/.aspera/connect/bin/ there you'll see the command line utilities where you're going to use 'ascp'.

 

Using the Aspera ascp command line program

Your command should look similar to this:    

ascp -P33001 -O33001 -QT -l300M L file.gpg  ega-box-N@fasp.ega.ebi.ac.uk:/. 

Explanation of parameters

'-l300M' option sets the upload speed limit to 30MB/s. You may wish to lower this value to increase the
reliability of the transfer.

'-L-' option is for printing logs out while transferring

<files to upload> can be a file mask (e.g. '/homes/submitter/*.srf) or a list of files.

<ega-box-N> is your submission account log-in.

Add '-k2' switch for transfer restarts

Check the command line transfer usage for more configuration details. 

 

Using FTP to upload your prepared files

FTP windows

FTP Linux/Unix

FTP client (Filezilla)

 

i) Using default ftp command line client in Windows

1-  Start the command line interpreter: press Win-R, type cmd, hit enter

2-  Enter 'ftp ftp.ega.ebi.ac.uk'

3-  Enter your submission username

4-  Enter your submission password

5- Type 'binary' to enter binary mode for transfer

6-  To see a list of available ftp commands type 'help'.

7-  Type 'ls' command to check the content of your submission account.

8-  Type 'prompt' to switch off confirmation for each file uploaded.

9-  Use 'mput' command to upload files: 'mput *.bam*'

10-  Use 'bye' command to exit the ftp client.

11-  Use 'exit' command to exit the command line interpreter.

 

ii) Using default ftp command line client in Linux/Unix

1-  Open a terminal and type 'ftp ftp.ega.ebi.ac.uk'

2-  Enter your submission username

3-  Enter your submission password

4- Type 'binary' to enter binary mode for transfer

5-  To see a list of available ftp commands type 'help'.

6-  Type 'ls' command to check the content of your drop box.

7-  Type 'prompt' to switch off confirmation for each file uploaded.

8-  Use 'mput' command to upload files: 'mput *.bam*'

9-  Use 'bye' command to exit the ftp client.

 

iii) Using an FTP client

e.g. Filezilla

Use the following connection details (File=> Site Manager) and add your submission account username and password :

 

Select the files you wish to upload, then right click mouse, and select 'upload':