DAC Accession Contact Person Email Access Information
EGAC00001000139 Etienne Patin epatin@pasteur.fr http://www.ebi.ac.uk/ega/dacs/EGAC00001000139

This DAC controls 7 datasets:

Dataset Accession Technologysort descending Samples Description
EGAD00010000690 160 Genome-wide SNP genotyping of African rainforest hunter-gatherers and neighbouring agriculturalists by Illumina HumanOmniExpress
EGAD00010000692 372 Genome-wide DNA methylation epigenotyping of African rainforest hunter-gatherers and neighbouring agriculturalists by Illumina HumanMethylation450
EGAD00001002714 Illumina HiSeq 2000;ILLUMINA 970 We recruited 100 healthy, male donors of self-reported European descent (EUB) and 100 of self-reported African descent (AFB) (Ghent, Belgium). For each participant, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood on Ficoll-Paque density gradients. Monocytes were then positively selected with magnetic CD14 microbeads and exposed for 6 hours to different ligands activating TLR4 (LPS), TLR1/2 (Pam3CSK4), TLR7/8 (R848) and to a human seasonal influenza A virus (IAV). High-quality RNA was obtained from unstimulated and stimulated monocytes for 970 of the 1000 samples (200 x 5 conditions), and was sequenced on an Illumina HiSeq2000. On average, 34 million 101-bp single-end reads were obtained per sample.
EGAD00010001251 Illumina HumanMethylation450 38 Epigenome of 36 rainforest hunther-gathering Baka of Cameroon by Illumina HumanMethylation450 array, used in the EGAS00001002226 study
EGAD00010000496 Illumina HumanOmni1-Quad-Illumina GenomeStudio 260 Genome-wide SNP genotyping of African rainforest hunter-gatherers and neighbouring agriculturalists
EGAD00010001131 Illumina HumanOmni5-Quad and exome sequencing 200 The 100 European-descent (EUB) and 100 African-descent (AFB) Belgians studied were genotyped for a total of 4,301,332 SNPs on the Illumina HumanOmni5-Quad BeadChips. Whole-exome sequencing was carried out for the same 200 individuals with the Nextera Rapid Capture Expanded Exome kit, on the Illumina HiSeq 2000 platform, with 100-bp paired-end reads. This kit delivers 62 Mb of genomic content per individual, including exons, untranslated regions (UTR), and microRNAs. Omni5 and exome datasets were merged, yielding a concordance rate between platforms of 99.93%.
EGAD00010001209 Illumina HumanOmniExpress-12 1235 Genome-wide SNP genotyping data for 1,235 western Africans by Illumina HumanOmniExpress-12 array, used in the EGAS00001002078 study

DAC description