Date: Saturday September 3, 2016
Time: 13:30 – 17:00 (Half day tutorial)
Venue: World Forum, room: Antarctica
This practical tutorial will present bioinformatic resources and computational tools to build and explore protein interactomes and sub-interactomes derived from physical interactions between protein pairs determined in multiple high-throughput or low-throughput experimental studies. The interactomes will be considered using quality assessment parameters and scores to assign confidence levels to the interactions. The tutorial will also show how to analyse the interactomes drawing sets of protein-protein interactions as networks in a relational context, by applying network-based tools and graph analytic methods to find modules and critical hubs (using Cytoscape and specific apps within this platform). As a particular case of study, the tutorial will focus on the exploration of the most recent comprehensive sets of the human interactome determined by a proteome-scale binary approach (using high-throughput two-hybrid technology) (Rolland et al. 2014, Cell; PMID: 25416956) or a systematic co-complex approach (using high- throughput affinity-purification mass spectrometry technology) (Huttlin et al. 2015, Cell; PMID: 26186194).
This will be a practical tutorial for biologists, molecular biologists or computational biologists, young researchers (mainly Master or PhD students) working in different areas of biomedical or biological sciences who are interested in the field of “network biology” and in learning –in a practical way– about bioinformatic resources and computational tools to build and explore protein interactomes and also to learn how to draw protein interactions as networks in a relational context using network-based tools.