Investigation Title	Transcription profiling of right ventricule from arrhythmogenic heterozygous plakoglobin deficient mice undergone endurance training to identify transcriptional responses to training.	
Comment[Submitted Name]	training induced gene expression in arrhythmogenic plakogloin (+/-) mice	
Experimental Design	stimulus_or_stress_design	individual_genetic_characteristics_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo		mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-11-06	
Comment[SecondaryAccession]	GSE4120	
Comment[AEMIAMESCORE]	3	
Comment[SecondaryAccession]	GDS1691	
Comment[ArrayExpressAccession]	E-GEOD-4120	
Comment[MAGETAB TimeStamp_Version]	2010-08-05 13:25:36 Last Changed Rev: 13058	
Experimental Factor Name	Genotype	Stress	
Experimental Factor Type	genotype	stress	
Experimental Factor Term Source REF	
Person Last Name	Witt	
Person First Name	Henning	
Person Mid Initials	
Person Email	witt@molgen.mpg.de	
Person Phone	
Person Fax	
Person Address	Patricia Ruiz, Hans Lehrach, Max Planck Institute for Molecular Genetics, Ihnestrasse 65, Berlin, 14195, Germany	
Person Affiliation	Max Planck Institute for Molecular Genetics	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-11-06	
PubMed ID	17030684	
Publication DOI	17030684	
Publication Author List	Paulus Kirchhof, Larissa Fabritz, Melanie Zwiener, Henning Witt, Michael Schäfers, Stephan Zellerhoff, Matthias Paul, Timur Athai, Karl-Heinz Hiller, Hideo A Baba, Günter Breithardt, Patricia Ruiz, Thomas Wichter, Bodo Levkau	
Publication Title	Age- and training-dependent development of arrhythmogenic right ventricular cardiomyopathy in heterozygous plakoglobin-deficient mice.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	Endurance training accelerated development of right ventricular dysfunction and arrhythmias in plakoglobin+/- mice. Histology and electron microscopy did not identify right ventricular abnormalities. To identify differences in the transcriptional response to training gene expression profiling was performed. Experiment Overall Design: Gene expressson analyses were performed in 5 pairs of plakoglobin (+/-) mice and their wildtype littermates. Four pairs of trained mice and one pair of old untrained mice were studied. Training induced expression of several hypertrophy associated genes such as ANP but no differences between the trained plakoglobin (+/-) mice and wt mice was seen.	
Protocol Name	P-G4120-9	P-G4120-2	P-G4120-1	P-G4120-4	P-G4120-5	P-G4120-6	P-G4120-8	
Protocol Type	grow	specified_biomaterial_action	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	standard animal care	training: mice were put into a 30 x 50, 20 cm deep container filled with warm (35 C) water equipped with a floating rotating wheel. Swimming duration was continously increased from 5 minutes to 90 minutes daily (6 times per week) swimming. Mice were stimulated to swim as sonn as they began floating to maximize activity in the water.	untrained	TRIzol Reagent (Invitrogen), standard protocol	The amplification and labeling of the RNA samples were carried out according to the manufacturer's instructions (Affymetrix, Santa Clara, CA). Briefly, total RNA was quantified by UV-spectroscopy and its quality was checked by analysis on a LabChip (BioAnalyzer, AGILENT Technologies, Santa Clara, CA). In the presence of an oligo(dT)24 primer containing a T7 RNA polymerase promoter (TIBMOL Biol, Berlin, Germany) one to three micrograms of total RNA were used for the synthesis of double-stranded cDNA (SuperScript transcriptase, Life Technologies, Inc., Carlsbad, CA). Labeled complementary RNA (cRNA) was prepared from double-stranded cDNA by in vitro transcription using the GeneChip RNA transcript labeling kit (Affymetrix, Santa Clara, CA). After cleanup (Qiagen, Hilden, Germany), the biotin-labeled cRNA was fragmented by alkaline treatment [40 mmol/L Tris-acetate (pH 8.2), 100 mmol//L potassium acetate, and 50 mmol//L magnesium acetate] at 94°C for 35 minutes.	15 ug of each cRNA sample was hybridized for 16 hours at 45°C to rat expression Affymetrix RAE 230A GeneChip arrays. Following the hybridization arrays were washed and stained with streptavidin-phycoerythrin using a fluidics station in accordance with the Affymetrix protocol.	Affymetrix GeneChip System confocal scanner 2500	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF							The MGED Ontology	
SDRF File	E-GEOD-4120.sdrf.txt	
Term Source Name		The MGED Ontology	EFO	mo	ncbitax	ArrayExpress	mo	EFO	The MGED Ontology	
Term Source File		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version