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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.

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Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London

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Protocols: Cells were plated in M3234 Methylcellulose medium supplemented with 20ng/ml SCF, 10ng/ml of each IL3, IL6 and GM-CSF and appropriate antibiotic selection on the following day. Colonies were scored after 7 days and replated every 7 days. 300ng to 1ug of mirVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.