# _embedded.samples[].characteristics.description[].text
NaÃ¯ve CD44-lo/CD62L-hi/CD8+ T cells purified from pooled CD8+ T cells from 2 normal C3H.SW mice.

# _embedded.samples[].characteristics.sample source name[].text
NaÃ¯ve CD44-lo/CD62L-hi/CD8+ T cells, biological replicate 2

# _embedded.samples[].characteristics.description[].text
Biological replicate 1 of 4. We printed our oligos onto two slides and the same sample was hybridized onto both slides. The Sample data tables contain merged normalized data from both slides.

# _embedded.samples[].characteristics.sample characteristics[].text
Undifferentiated rice (c.v. Nipponbare) suspension cultured cells

# _embedded.samples[].characteristics.sample source name[].text
Amplified RNA from rice suspension cultured cells (red).

# _embedded.samples[].characteristics.description[].text
Biological replicate 3 of 4. We printed our oligos onto two slides and the same sample was hybridized onto both slides. The Sample data tables contain merged normalized data from both slides.

# _embedded.samples[].characteristics.sample characteristics[].text
Rice (c.v. Nipponbare) Seedling - Root maturation zone - Stele/Vascular bundle from 5 day old seedlings grown in an environmental chamber in a short day cycle of 10hr light of 120ÂµE/m^2/sec at 30Â°C and 14hr darkness at 24Â°C.

# _embedded.samples[].characteristics.sample source name[].text
Amplified RNA from rice Seedling - Root maturation zone - Stele/Vascular bundle (red)

# _embedded.samples[].characteristics.description[].text
Biological replicate 2 of 4. We printed our oligos onto two slides and the same sample was hybridized onto both slides. The Sample data tables contain merged normalized data from both slides.

# _embedded.samples[].characteristics.sample characteristics[].text
Rice (c.v. Nipponbare) Seedling - Leaf blade (2nd leaf) - Bundle sheath from 5 day old seedlings grown in an environmental chamber in a short day cycle of 10hr light of 120ÂµE/m^2/sec at 30Â°C and 14hr darkness at 24Â°C.

# _embedded.samples[].characteristics.sample source name[].text
Amplified RNA from rice Seedling - Leaf blade (2nd leaf) - Bundle sheath (green)

# _embedded.samples[].characteristics.sample source name[].text
Heads of WAG0 pea aphid reared under 16h photoperiod

# _embedded.samples[].characteristics.description[].text
Biological replicate 1 of 4. We printed our oligos onto two slides and the same sample was hybridized onto both slides. The Sample data tables contain merged normalized data from both slides.

# _embedded.samples[].characteristics.sample characteristics[].text
Undifferentiated rice (c.v. Nipponbare) suspension cultured cells

# _embedded.samples[].characteristics.sample source name[].text
Amplified RNA from rice suspension cultured cells (red).

# _embedded.samples[].characteristics.description[].text
Genotype and allele specfic intensity of leukocyte DNA

# _embedded.samples[].characteristics.sample source name[].text
Heads of WAG0 pea aphid reared under 12h photoperiod

# _embedded.samples[].characteristics.submission description[].text
The insulin-like signaling (ILS) pathway regulates metabolism and is known to modulate adult lifespan in C. elegans. Altered stress responses and resistance to a wide range of stressors are also associated with changes in ILS and contribute to enhanced longevity. The transcription factors DAF-16 and HSF-1 are key effectors of the longevity phenotype. We demonstrate that increased intrinsic thermotolerance, due to lower ILS, is not dependent on stress induced HSF-1 transcriptional responses but instead requires active protein translation. Translation profiling experiments reveal genes that are post-transcriptionally regulated in response to altered ILS during heat shock in a DAF-16-dependent manner. Furthermore, several novel proteins are specifically required for ILS effects on thermotolerance. We propose that lowered-ILS results in DAF-16-induced metabolic and physiological changes that precondition a translational response to modulated survival under acute stress. 72 experimental samples were analyzed using custom oligo microarrays. A wild type sample pool was used as the Cy3 reference/control for all experimetal samples. All extracted RNA prior to array analysis was fractioned (via a sucrose gradient) based on ribosomal loading and pooled into ribosomal and free RNA (F1), light polysomes (F2) and heavy polysomes (F3) as described in the experimental procedures. The control RNAi is ‘empty’ vector L4440 RNAi feeding vector plasmid (1999 Firelab vector kit) transformed HT115(DE3), which was obtained from the Caenorhabditis Genetics Center (University of Wisconsin).

# _embedded.samples[].characteristics.submission title[].text
Insulin-like Signaling Determines Survival During Stress via Post Transcriptional Mechanisms in C. elegans.

# _embedded.samples[].characteristics.description[].text
Genotype and allele specfic intensity of leukocyte DNA