# _embedded.samples[].characteristics.sample source name[].text
Commercial pools of healthy DNA (Promega, Madison, WI, USA)

# _embedded.samples[].characteristics.sample source name[].text
Commercial pools of healthy DNA (Promega, Madison, WI, USA)

# _embedded.samples[].characteristics.sample source name[].text
Skin tumor, tumor-stage mycosis fungoides

# _embedded.samples[].characteristics.sample source name[].text
Skin tumor, tumor-stage mycosis fungoides

# _embedded.samples[].characteristics.source prep[].text
Skeletal muscle injured with cardiotoxin 4 days before cell isolation. Collagenase digestion-Percoll- sorting in Moflo

# _embedded.samples[].characteristics.sample source name[].text
Commercial pools of healthy DNA (Promega, Madison, WI, USA)

# _embedded.samples[].characteristics.sample source name[].text
Skin tumor, tumor-stage mycosis fungoides

# _embedded.samples[].characteristics.sample source name[].text
Commercial pools of healthy DNA (Promega, Madison, WI, USA)

# _embedded.samples[].characteristics.description[].text
Biological replicate 1 of 4. We printed our oligos onto two slides and the same sample was hybridized onto both slides. The Sample data tables contain merged normalized data from both slides.

# _embedded.samples[].characteristics.sample characteristics[].text
Rice (c.v. Nipponbare) embryo scutellum from germinated seeds (24h post-imbibition).

# _embedded.samples[].characteristics.sample source name[].text
Amplified RNA from rice Embryo - Scutellum - 24hr post imbibition (green)

# _embedded.samples[].characteristics.sample source name[].text
Skin tumor, tumor-stage mycosis fungoides

# _embedded.samples[].characteristics.description[].text
The control RNAi is ‘empty’ vector L4440 RNAi feeding vector plasmid (1999 Firelab vector kit) transformed HT115(DE3). The empty vector RNAi was used as the food source for our array experiment.

# _embedded.samples[].characteristics.sample source name[].text
daf16(M26); daf-2(e1370) Heat Shocked on Control RNAi

# _embedded.samples[].characteristics.source prep[].text
Skeletal muscle injured with cardiotoxin 14 days before cell isolation. Collagenase digestion-Percoll- sorting in Moflo

# _embedded.samples[].characteristics.description[].text
The control RNAi is ‘empty’ vector L4440 RNAi feeding vector plasmid (1999 Firelab vector kit) transformed HT115(DE3). The empty vector RNAi was used as the food source for our array experiment.

# _embedded.samples[].characteristics.source prep[].text
Skeletal muscle injured with cardiotoxin 14 days before cell isolation. Collagenase digestion-Percoll- sorting in Moflo

# _embedded.samples[].characteristics.submission description[].text
NaÃ¯ve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison 9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.

# _embedded.samples[].characteristics.submission title[].text
Comparison of naÃ¯ve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice

# _embedded.samples[].characteristics.description[].text
Genotype and allele specfic intensity of leukocyte DNA

# _embedded.samples[].characteristics.description[].text
donor CD44-lo/CD62L-hi/CD8+ T cells from normal C3H.SW mice were transplanted together with T cell-depleted bone marrow into 4 lethally irradiated (10Gy) B6/SJL recipient mice to induce GVHD. After 14 days CD44-hi/CD62L-lo/CD8+ T cells were purified from pooled T cells from the recipients.

# _embedded.samples[].characteristics.sample source name[].text
CD44-hi/CD62L-lo/CD8+ effector memory T cells, biological replicate 2