# _embedded.samples[].characteristics.sample source name[].text
Mouse thioglycollate-elicited peritoneal macrophages stimulated with 10ng/ml S. minnesota lipopolysaccharide for 24h

# _embedded.samples[].characteristics.sample source name[].text
brain, total RNA from 3 ethanol-treated males

# _embedded.samples[].characteristics.sample source name[].text
brain, total RNA from 3 saline-treated males

# _embedded.samples[].characteristics.sample source name[].text
Mouse thioglycollate-elicited peritoneal macrophages stimulated with 10ng/ml S. minnesota lipopolysaccharide for 24h

# _embedded.samples[].characteristics.description[].text
total RNA was harvested from 4 x 106 cells using a High Pure RNA isolation kit (Roche Applied Science) according to manufacturer's instructions. For xenografts, tumors were were excised 22-29 days post-implantation under accredited procedures (Piedmont Research Center, Morrisville, NC), snap-frozen in liquid nitrogen and stored at -80 degrees C until use. Total RNA was prepaed from frozen specimens by 24 hr immersion at -80 degrees C in RNAlater-ICE (Ambion) to 'transition' solid tumors for subsequent homogenization by grinding with a liquid nitrogen-chilled mortar/pestle, followed by resuspension in Trizol.

# _embedded.samples[].characteristics.description[].text
Gene expression data from UCSD CLL patient #27

# _embedded.samples[].characteristics.description[].text
Gene expression data from UCSD CLL patient #19

# _embedded.samples[].characteristics.description[].text
Gene expression data from UCSD CLL patient #109

# _embedded.samples[].characteristics.description[].text
cells were grown with 10% fetal calf serum in Dulbecco's modified Eagle's medium at 37 degrees C in 5% C02

# _embedded.samples[].characteristics.description[].text
cDNA from parental cells, Human Universal RNA and xenografts was generated with amino-allyl dUTP using a modified Agilent Fluorescent Direct Label kit. The cDNA was purified with QIAquick PCR columns and coupled to either N-hydroxysuccinimidyl-esterified Cy3 or Cy5 dyes. Dye-conjugated cDNAs were purified from free dye using the CyScribe GFX purification kit (Amersham). Arrays were hybridized for 16 hr at 60 degrees C using an Agilent In Situ Hybridization kit.