# _embedded.samples[].characteristics.cell type[].text
mixture of liver, testis, mammary gland, cervix, brain, skin, liposarcoma, macrophage, T-lymphoblast and B-lymphocyte

# _embedded.samples[].characteristics.cell type[].text
FACS-sorted GFP positive cells from single cell suspensions of embryos

# _embedded.samples[].characteristics.description[].text
FACS-sorted GFP positive cells from single cell suspensions of embryos with TinD-GFP insertions targeting GFP to Tinman-expressing cardiac mesodermal cells staged to late stage 11/early stage 12

# _embedded.samples[].characteristics.description[].text
Gene expression data from cerebral cortex

# _embedded.samples[].characteristics.description[].text
Gene expression data from cerebral cortex

# _embedded.samples[].characteristics.submission description[].text
The development of a complex organ requires the specification of appropriate numbers of each of its constituent cell types, as well as their proper differentiation and correct positioning relative to each other. During Drosophila cardiogenesis, all three of these processes are controlled by jumeau (jumu) and Checkpoint suppressor homologue (CHES-1-like), two genes encoding forkhead transcription factors that we discovered utilizing an integrated genetic, genomic and computational strategy for identifying novel genes expressed in the developing Drosophila heart. Both jumu and CHES-1-like are required during asymmetric cell division for the derivation of two distinct cardiac cell types from their mutual precursor, and in symmetric cell divisions that produce yet a third type of heart cell. jumu and CHES-1-like control the division of cardiac progenitors by regulating the activity of Polo, a kinase involved in multiple steps of mitosis. This pathway demonstrates how transcription factors integrate diverse developmental processes during organogenesis. GFP-positive and GFP-negative cells were profiled from Stage 11 TinD-GFP Drosophila embryos

# _embedded.samples[].characteristics.submission title[].text
Two Forkhead transcription factors regulate the division of cardiac progenitor cells by a Polo-dependent pathway

# _embedded.samples[].characteristics.description[].text
Gene expression data from cerebral cortex

# _embedded.samples[].characteristics.description[].text
Gene expression data from cerebral cortex

# _embedded.samples[].characteristics.submission description[].text
Transcriptome analysis of RNAs extracted from early passage and late passage (immortalized) mouse embryonic fibroblasts (MEFs) We compared the gene expression profiles of early (p.6-7) and immortalized (p.28-33) Smurf2KO cells to those of their wild-type counter parts, and selected the genes that were differentially expressed at a cut-off of 1.54 folds in both B6 and BL backgrounds. The results showed a progressive increase in differential gene activities with the number of cell passages. We detected 101 differentially expressed genes in the early passage and 668 in the immortalized Smurf2KO cells, with 49 in common between these two categories. These data pointed to a global deregulation of gene expression as the cells became immortalized in the absence of Smurf2. We analyzed MEFs from wild type and Smurf2-/- embryos from both C57B6/L (B6) and mixed 129SVJ x NIH Black Swiss (BL) backgrounds using the Affymetrix mouse Exon 1.0 ST platform. No techinical replicates were performed.

# _embedded.samples[].characteristics.submission title[].text
Gene Level Expression Profiling of MEFs derived from wild type and Smurf2-/- embryos

# _embedded.samples[].characteristics.description[].text
Gene expression from mice infested for 3 hrs

# _embedded.samples[].characteristics.sample title[].text
Tick-infested mouse, 3 hour after tick attachment, biological rep3

# _embedded.samples[].characteristics.description[].text
Gene expression profiling data from cerebellum

# _embedded.samples[].characteristics.sample source name[].text
cerebellum from Npc1 (BALB/cNctr-Npc1m1N/J) mice