# _embedded.samples[].characteristics.disease state[].text
germinal center B-cell like diffuse large B-cell lymphoma

# _embedded.samples[].organization[].Name
King Abdullah University of Science and Technology

# _embedded.samples[].characteristics.cell type[].text
mixture of liver, testis, mammary gland, cervix, brain, skin, liposarcoma, macrophage, T-lymphoblast and B-lymphocyte

# _embedded.samples[].characteristics.sample source name[].text
Universal Human Reference RNA, cy5-r.fl.

# _embedded.samples[].characteristics.submission description[].text
Overnutrition during pregnancy inﬂuences the future health of the offspring, with outcomes differing depending on the child’s sex. The placenta is involved in the programming of obesity, type 2 diabetes and cardiovascular disease. Sex-specific adaptation of the placenta may be central to the differences in fetal growth and survival. The impact of diet and fetal sex on placental gene expression and epigenetic marks was investigated in mice fed a high-fat (HFD) or a control diet (CD), during the first 15 days of gestation Microarrays analysis revealed that expression was affected by maternal diet and was sexually dimorphic. We analyzed the placentae of 4 mice litters fed with an high-fat diet (HFD) and 4 with a control diet (CD). For each litter, the placenta transcriptome was analysed according to the foetus sex using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. No technical replicates were performed.

# _embedded.samples[].characteristics.submission title[].text
Female- and male-specific transcriptomic and epigenetic placental signatures in response to a maternal high fat diet in mice

# _embedded.samples[].organization[].Name
Université Paris Descartes - Hopital Necker-Enfants Malades

# _embedded.samples[].organization[].Name
Université Paris Descartes - Hopital Necker-Enfants Malades

# _embedded.samples[].characteristics.submission description[].text
PRDM proteins belong to the SET domain protein family, which is involved in the regulation of gene expression. Although few PRDM members possess histone methyltransferase activity, the molecular mechanisms by which the other members exert transcriptional regulation remain to be delineated. In this study, we find that Prdm5 is highly expressed in mouse embryonic stem (mES) cells and exploit this cellular system to characterize molecular functions of Prdm5. By combining proteomics and next-generation sequencing technologies, we identify Prdm5 interaction partners and genomic occupancy. We demonstrate that although Prdm5 is dispensable for mES cell maintenance, it directly targets genomic regions involved in early embryonic development and affects the expression of a subset of developmental regulators during cell differentiation. Importantly, Prdm5 interacts with Ctcf, cohesin, and TFIIIC and cooccupies genomic loci. In summary, our data indicate how Prdm5 modulates transcription by interacting with factors involved in genome organization in mouse embryonic stem cells. For each condition (ATRA-induced differentiation model and LIF cytokine deprivation) three replicate are available for both Prdm5 wt mES cells and Prdm5 KO mES cells, for a total of 12 samples

# _embedded.samples[].characteristics.submission title[].text
Genomic and Proteomic Analyses of Prdm5 Reveal Interactions with Insulator Binding Proteins in Embryonic Stem Cells (RNA-seq)

# _embedded.samples[].characteristics.cell type[].text
mixture of liver, testis, mammary gland, cervix, brain, skin, liposarcoma, macrophage, T-lymphoblast and B-lymphocyte

# _embedded.samples[].characteristics.sample source name[].text
Universal Human Reference RNA, cy5-r.fl.

# _embedded.samples[].characteristics.cell type[].text
mixture of liver, testis, mammary gland, cervix, brain, skin, liposarcoma, macrophage, T-lymphoblast and B-lymphocyte

# _embedded.samples[].characteristics.submission description[].text
Series of 4 repetitions of hybridization of treatment (tic40) and control (WT) each. Comparison of the Arabidopsis tic40 mutant exhibiting an impaired protein import across the inner chloroplast envelope vs WT. L. Heins et al., EMBO J 21 (2002), pp. 2616-2625 Keywords: repeat sample

# _embedded.samples[].characteristics.submission description[].text
We used LCM and microarrays to analyze the transcriptomes of three CVOs, the SCO, the subfornical organ (SFO), and the PG, and the third ventricle ependyma in the adult rat in order to better characterize these organs at the molecular level. Gene expression was performed on laser microdissection of rat Pineal Gland (n=2), Subcommisural Organ (pool of 2), Subfornical Organ (pool of 2) and Third Ventricle Ependyma (pool of 2). Pool of rat total normal brains was used as reference.

# _embedded.samples[].characteristics.submission title[].text
Molecular Characterization of Laser Microdissected Circumventricular Organs and Third Ventricle Ependyma in the Adult Rat by Microarray Analysis.