# _embedded.samples[].characteristics.description[].text
channel ch1 is ChIP DNA; Antibody information listed below: official name: H3K4me3 (ab8580 lot1);target name: H3K4me3;host: Rabbit;antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3 tri methylated at K4.;clonal: Polyclonal;purified: Affinity;company: Abcam;catalog: 8580;reference: http://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-ChIP-Grade-ab8580.html;short description: Histone H3 (tri methyl K4).;

# _embedded.samples[].characteristics.submission description[].text
Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth; and development of plants. BR signaling through cell-surface receptor; kinases and intracellular components leads to dephosphorylation and; accumulation of the nuclear protein BZR1. How BR signaling regulates gene; expression, however, remains unknown. Here we show that BZR1 is a; transcriptional repressor that has a previously unknown DNA binding domain; and binds directly to the promoters of feedback-regulated BR biosynthetic; genes. To identify additional BZR1-regulated genes and to understand the; BR-regulated transcriptional pathways, we examined the effects of bzr1-1D; and det2 mutations on the expression of BR-regulated genes by using the; Arabidopsis full-genome oligo microarray (Affymetrix). Microarray analyses; identified additional potential targets of BZR1 and illustrated, together; with physiological studies, that BZR1 coordinates BR homeostasis and; signaling by playing dual roles in regulating BR biosynthesis and downstream; growth responses.

# _embedded.samples[].characteristics.submission title[].text
Transcription profiling of Arabidopsis Brassinosteroid mutant (bzr1-1D and det2) analysis

# _embedded.samples[].characteristics.description[].text
Hybridizations conducted using Balance Interwoven Loop Design including 2 technical replicates per treatment and 2 dye swaps.

# _embedded.samples[].characteristics.description[].text
Hybridizations conducted using Balance Interwoven Loop Design including 2 technical replicates per treatment and 2 dye swaps.

# _embedded.samples[].characteristics.description[].text
Hybridizations conducted using Balance Interwoven Loop Design including 2 technical replicates per treatment and 2 dye swaps.

# _embedded.samples[].characteristics.cells[].text
normal human osteoblasts sample A2 plus doping controls.

# _embedded.samples[].characteristics.sample source name[].text
NH Osteoblasts sample A2 plus doping controls.

# _embedded.samples[].characteristics.description[].text
Hybridizations conducted using Balance Interwoven Loop Design including 2 technical replicates per treatment and 2 dye swaps.

# _embedded.samples[].characteristics.cells[].text
normal human osteoblasts and MCF-7 coculture B plus doping controls.

# _embedded.samples[].characteristics.sample source name[].text
NH Osteoblasts and MCF-7 coculture B plus doping controls.

# _embedded.samples[].characteristics.description[].text
Hybridizations conducted using Balance Interwoven Loop Design including 2 technical replicates per treatment and 2 dye swaps.