# _embedded.samples[].organization[].Name
Oregon National Primate Research Center - OHSU

# _embedded.samples[].characteristics.submission description[].text
The model cyanobacterium Synechocystis sp. PCC 6803 was used for a systematic survey on the number and types of antisense (as)RNAs. A tiled microarray was constructed with probes for both strands of the genes or intergenic spacers with candidate transcripts predicted by an algorithm and an equally sized and distributed control set. The resulting 102,739 individual probes cover an accumulated length of 1,441,146 nt or about 40% of the total chromosome sequence of Synechocystis 6803. Hybridization of this array with total RNA isolated from cultures raised under different growth conditions identified a high number of transcripts from intergenic spacers and in antisense orientation to known genes (natural cis-asRNAs). Extrapolated to the whole genome, around 10% of all open reading frames in Synechocystis 6803 may have a corresponding asRNA, suggesting a much more important role of chromosomally encoded asRNAs in bacteria then anticipated so far. Keywords: stress response 4 RNA hybridizations, 2 DNA controls. The raw data from the DNA hybridizations are in the GSE14410_DNA_1.txt and GSE14410_DNA_2.txt files.

# _embedded.samples[].characteristics.submission title[].text
Evidence for a major role of antisense RNAs in cyanobacterial gene regulation

# _embedded.samples[].characteristics.description[].text
This sample is of Antirrhinum majus cv. Maryland True Pink petal tissue harvested the day of anthesis. It is the second of three biological replicates used in this experiment.

# _embedded.samples[].characteristics.sample source name[].text
Antirrhinum majus cv. Maryland True Pink petal d1

# _embedded.samples[].characteristics.sample characteristics[].text
non-treated = reference = time point zero

# _embedded.samples[].characteristics.sample source name[].text
Rhodopirellula baltica cold shock reference

# _embedded.samples[].organization[].Name
Max Planck Institut for Marine Microbiology

# _embedded.samples[].characteristics.description[].text
Epithelial ovarian cancers primary_data_description: Log relative ratio calclulated by Affymetric Genotyping Console

# _embedded.samples[].characteristics.sample characteristics[].text
non-treated = reference = time point zero

# _embedded.samples[].characteristics.sample source name[].text
Rhodopirellula baltica cold shock reference

# _embedded.samples[].organization[].Name
Max Planck Institut for Marine Microbiology

# _embedded.samples[].characteristics.description[].text
Colonic mucosa was sheared by microscope slide and flash frozen on dry ice

# _embedded.samples[].characteristics.sample source name[].text
Whole female mosquito sugar-fed 3 days post-emergence

# _embedded.samples[].characteristics.description[].text
Epithelial ovarian cancers primary_data_description: Log relative ratio calclulated by Affymetric Genotyping Console

# _embedded.samples[].characteristics.sample title[].text
Uveal melanoma from enucleation 51 [UCSF]

# _embedded.samples[].organization[].Name
Washington University School of Medicine

# _embedded.samples[].characteristics.sample source name[].text
Rhodopirellula baltica, cold shock, 40min

# _embedded.samples[].organization[].Name
Max Planck Institut for Marine Microbiology

# _embedded.samples[].characteristics.description[].text
Epithelial ovarian cancers primary_data_description: Log relative ratio calclulated by Affymetric Genotyping Console

# _embedded.samples[].characteristics.sample characteristics[].text
non-treated = reference = time point zero

# _embedded.samples[].characteristics.sample source name[].text
Rhodopirellula baltica cold shock reference

# _embedded.samples[].organization[].Name
Max Planck Institut for Marine Microbiology