# _embedded.samples[].characteristics.sample title[].text
cytoplasmic RNA after M transfection (16h) and DMSO treatment(24h) rep1

# _embedded.samples[].characteristics.sample title[].text
nuclear RNA after M transfection (16h) and DMSO treatment(24h) rep2

# _embedded.samples[].characteristics.sample title[].text
nuclear RNA after M transfection (16h) and DMSO treatment(24h) rep1

# _embedded.samples[].characteristics.sample title[].text
total RNA after M transfection (16h) and DMSO treatment(24h) rep2

# _embedded.samples[].characteristics.sample source name[].text
bone marrow-derived mononuclear cells from OA patient

# _embedded.samples[].characteristics.sample characteristics[].text
chip antibody cat. #: A2220 chip antibody lot #: 069K6094

# _embedded.samples[].characteristics.submission description[].text
Asc1p and its essential orthologues in higher eukaryotes, as e.g. RACK1 in mammals, are involved in several distinct cellular signaling processes, but the implications of a total deletion have never been assessed in a comprehensive genome-wide manner. This study reveals the major cellular processes affected in a Saccharomyces cerevisiae Δasc1 deletion background via de novo proteome and transcriptome analysis, as well as subsequent phenotypical characterizations. The deletion of ASC1 reduces iron-uptake and causes nitrosative stress, both known indicators for hypoxia, which manifests in a shift of energy metabolism from respiration to fermentation in the Δasc1 strain. The impact of Asc1p in cellular metabolism is further expressed by its regulative role in the MAP kinase signal transduction pathways of invasive/filamentous growth, pheromone response and cell wall integrity. In the Δasc1 mutant strain aberrations from the expected cellular response, mediated by these pathways, can be observed and are linked to changes in protein abundances of pathway-targeted transcription factors. Evidence for the translational regulation of such transcription factors suggests that ribosomal Asc1p is involved in signal transduction pathways by controlling the biosynthesis of the respective final transcriptional regulators. The transcriptional data results from from 3 biological replicates for each strain, whereas for each biological replicate 2 technical replications and dye-swaps were analyzed.

# _embedded.samples[].characteristics.submission title[].text
Comparative transcriptome analysis of Saccharomyces cerevisiae wild type and Δasc1 strain

# _embedded.samples[].characteristics.submission description[].text
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

# _embedded.samples[].characteristics.submission title[].text
Distribution, recognition and regulation of non-CpG methylation in the adult mammalian brain

# _embedded.samples[].characteristics.sample source name[].text
nuclear RNA_M transfection_compound 1 (5uM)

# _embedded.samples[].characteristics.sample title[].text
nuclear RNA after M transfection (16h) and compound 1(5uM) treatment(24h) rep2

# _embedded.samples[].characteristics.sample source name[].text
total RNA_empty vector transfection_compound 1 (5uM)

# _embedded.samples[].characteristics.sample title[].text
total RNA after empty vector transfection (16h) and compound 1(5uM) treatment(24h) rep1

# _embedded.samples[].characteristics.submission description[].text
Small noncoding RNAs (sncRNA) are becoming recognized for their participation in a diverse range of cellular functions. In this study, their global characterization in human spermatozoa from donors with proven fertility was undertaken. Reads were analyzed in two classes, those mapping to unique locations and those that could be aligned to up to 10 genomic locations. All libraries showed comparable distribution of reads between intergenic, intronic and exonic genomic regions. Analysis of the sequences revealed the presence of multiple small RNA classes. The miRNAs retained in spermatozoa were found to be associated with promoter regions, suggestive of a role at the transcriptional level. Piwi-interacting sncRNAs as well as repeat-associated small RNAs were identified for the first time in mature spermatozoa from a mammalian species. Human spermatozoa retain a multifaceted population of small non-coding RNAs. Their presence is consistent with the view that they may function to stabilize the genome as part of the confrontation and consolidation of the genomes at fertilization. Examination of sperm samples from 3 individuals, sequenced individually

# _embedded.samples[].characteristics.submission title[].text
Characterization of the small RNA population in Human spermatozoa