# characteristics.description[].text
Protocols: Peritoneal membrane was harvested at 3h after the induction of peritoneal inflammation. Splenic naïve CD4+ T-cells were flow sorted and place in coated plates with anti-CD3e and 5μg/ml soluble anti-CD28 antibodies. Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNY-secreting CD4+ T-cells (Th1 cells) was determined by intracellular flow cytometry using antibodies against to CD4, IFNY, IL-17, IL-4 and IL-13. Based on this analysis, T-cells were washed in ice cold PBS, re-suspended in a 500μl aliquot of PBS reconstituted SES, and administered via the intra-peritoneal route. Tissues were diced and ground to a fine powder with intermittent addition of liquid nitrogen. Omni-ATAC seq was performed as described previously (Corces et al., 2017). Briefly, 100.000 nuclei per sample were isolated using an iodixanol gradient. ATAC-seq was performed according to the original protocol (Buenrostro et al., 2013) using Nextera DNA Sample preparation kit (Illumina, FC-121-1030).