# characteristics.Submitter Id[].tag
Namespace:ERASMUS MC, UNIVERISTY MEDICAL CENTER

# characteristics.description[].text
A SARS-CoV-2 specific multiplex PCR for Nanopore sequencing was performed, similar to amplicon-based approaches as previously described. In short, primers for 86 overlapping amplicons spanning the entire genome were designed using primal. The amplicon length was set to 500bp with 75bp overlap between the different amplicons. The libraries were generated using the native barcode kits from Nanopore (EXP-NBD104 and EXP-NBD114 and SQK-LSK109) and sequenced on a R9.4 flow cell multiplexing up to 24 samples per sequence run. Raw data was demultiplexed, amplicon primers were trimmed and human data was removed by mapping against the human reference genome.

# characteristics.organism[].text
Severe acute respiratory syndrome coronavirus 2

# characteristics.sample capture status[].text
active surveillance in response to outbreak

# characteristics.scientific_name[].text
Severe acute respiratory syndrome coronavirus 2

# characteristics.title[].text
Dutch COVID-19 response sample sequencing