# characteristics.description[].text
Protocols: FACS sorted subsets were either left untouched (Ex vivo) or cultured for 6h with 1mg/ml LPS and 10mg/ml anti-IgM, before DNA extraction. ~40,000 cells were pelleted by centrifuging for 5 min at 4 °C at 300 x g. After centrifugation, the pellet was carefully resuspended in the transposase reaction mix (12.5 ml 2xTD buffer, 2 ml TDE1 (Illumina) and 10.25 ml nuclease-free water, 0.25 µl 5% Digitonin (Sigma)) for 30 min at 37 °C. Per manufacturer's instruction Per manufacturer's instruction