# characteristics.sample characteristics[].text
FNA (fine needle aspiration biopsy) of melanoma metastases were obtained pre-therapy. A 23 Gauge hypodermic needle was inserted into the tumor. After insertion, multiple passes at changing angles of insertion were made through all quadrants of the tumor. Tissue collected into the needle was suspended in 10 ml cold RPMI and centrifuged at 1500 rpm for 5 minutes at 4oC. The cell pellet was harvested and red blood cells were eliminated with ACK lysis buffer. Cells were resuspended in 1 ml cold RPMI, transferred to a 1.5 ml Eppendorf tube, spun at 1500 rpm for 5 minutes and the supernatant was discarded. The cell pellet was resuspended in 500 ul of Trizol (Gibco BRL) and mixed thoroughly in the lysis buffer to ensure lysis of all cells. Lysates were frozen at -80oC until use.

# characteristics.sample title[].text
Patient 10 -Pre-therapy Dose 0 -tumor -repeat 2 - mAdbID:107512