# _embedded.samples[].characteristics.submission description[].text
Niemann-Pick Type C disease is an autosomal recessive neurodegenerative disorder with abnormal lipid storage as the major cellular pathologic hallmark. Genetic analyses have identified mutations in NPC1 gene in the great majority of cases, while mutations in NPC2 account for the remainders. Yet, little is known regarding the cellular mechanisms responsible for NPC pathogenesis, especially for neurodegeneration, which is the usual cause of death. To identify critical steps that could account for the pathological manifestations of the disease in one of the most affected brain structures, we performed global gene expression analysis in the cerebellum from three-week old Npc1+/+ and Npc1-/- mice with two different microarray platforms (Agilent and Illumina). Our results provide novel molecular insight regarding the mechanisms of pathogenesis in NPC disease and reveal potential new therapeutic targets. We performed global gene expression analysis in the cerebellum from three-week old Npc1+/+ and Npc1-/- mice with two different microarray platforms (Agilent and Illumina). Differentially-expressed genes identified by both microarray platforms were then subjected to KEGG pathway analysis. Expression of genes in six pathways was significantly altered in Npc1-/- mice; functionally, these signaling pathways belong to the following three categories: 1) steroid and terpenoid biosynthesis, 2) immune response, and 3) cell adhesion/motility. In addition, the expression of several proteins involved in lipid transport was significantly altered in Npc1-/- mice.

# _embedded.samples[].characteristics.submission title[].text
Microarray analysis in Npc1-/- mouse cerebellum

# _embedded.samples[].characteristics.sample characteristics[].text
Purified B cells from healthy human donor

# _embedded.samples[].characteristics.sample title[].text
2-phenylethyl ITC spiked - EC10 vs. Acetone Control EC10-2_AC4_F.candida_2-phenITC

# _embedded.samples[].characteristics.sample type[].text
Pool of approximately thirty animals of F.candida ('Berlin' Strain), age 25 days old, exposed for 2 days in a jar containing LUFA 2.2 soil spiked with only acetone (acetone control).

# _embedded.samples[].characteristics.description[].text
Gene expression profiling data from cerebellum

# _embedded.samples[].characteristics.sample source name[].text
cerebellum from Npc1 (BALB/cNctr-Npc1m1N/J) mice

# _embedded.samples[].characteristics.description[].text
Gene expression analysis of pollen-pistil interactions in Arabidopsis

# _embedded.samples[].characteristics.description[].text
Gene expression from mice infested for 1hr

# _embedded.samples[].characteristics.sample title[].text
Tick-infested mouse, 1 hour after tick attachment, biological rep3

# _embedded.samples[].organization[].Name
Ospedale Maggiore IRCCS - University of Milan

# _embedded.samples[].characteristics.description[].text
channel ch2 is negative control for ChIP; Antibody information listed below: official name: No Antibody Control;target name: Not Applicable;host: None-Control;antigen: NONE;clonal: None-Control;purified: None-Control;company: None-Control;catalog: Not Applicable;reference: Not Applicable;short description: Specific Antibodies were omitted from this immunoprecipitation reaction (as a negative control).;

# _embedded.samples[].characteristics.sample characteristics[].text
Purified T cells from healthy human donor

# _embedded.samples[].characteristics.description[].text
Gene expression profiling data from cerebellum

# _embedded.samples[].characteristics.sample source name[].text
cerebellum from Npc1 (BALB/cNctr-Npc1m1N/J) mice

# _embedded.samples[].characteristics.description[].text
Gene expression analysis of pollen-pistil interactions in Arabidopsis

# _embedded.samples[].characteristics.sample characteristics[].text
TTHA1359 deletion mutant strain grown on a rich (TR) medium for 680 min

# _embedded.samples[].characteristics.sample source name[].text
T. thermophilus HB8, TTHA1359 deletion mutant, rich medium, 680 min

# _embedded.samples[].characteristics.sample characteristics[].text
Purified B cells from healthy human donor

# _embedded.samples[].characteristics.description[].text
channel ch2 is negative control for ChIP; Antibody information listed below: official name: No Antibody Control;target name: Not Applicable;host: None-Control;antigen: NONE;clonal: None-Control;purified: None-Control;company: None-Control;catalog: Not Applicable;reference: Not Applicable;short description: Specific Antibodies were omitted from this immunoprecipitation reaction (as a negative control).;

# _embedded.samples[].organization[].Name
Ospedale Maggiore IRCCS - University of Milan