# characteristics.description[].text
Protocols: We have isolated clonal immortalized preadipocyte lines from stromal vascular fractions (SVFs) that were obtained from supraclavicular biopsies of 2 individuals undergoing surgery (30- and 43-years old). RiboZol reagent (AMRESCO) following the manufacturer's protocol Ultralow DR library kit (NuGEN): www.nugeninc.com/nugen/index.cfm/products/cs/ngs/rna-seq-v2