Caydasi2012 - Regulation of Tem1 by the GAP complex in spindle position cell cycle checkpoint - Ubiquitous association model

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Model Identifier
BIOMD0000000699
Short description
Caydasi2012 - Regulation of Tem1 by the GAP complex in spindle position cell cycle checkpoint - Ubiquitous association model

This model is described in the article:

Caydasi AK, Lohel M, Grünert G, Dittrich P, Pereira G, Ibrahim B.
Mol. Syst. Biol. 2012; 8: 582

Abstract:

The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1-Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1-Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1-Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition.

This model is hosted on BioModels Database and identified by: BIOMD0000000699.

To cite BioModels Database, please use: Chelliah V et al. BioModels: ten-year anniversary. Nucl. Acids Res. 2015, 43(Database issue):D542-8.

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Format
SBML (L2V4)
Related Publication
  • A dynamical model of the spindle position checkpoint.
  • Caydasi AK, Lohel M, Grünert G, Dittrich P, Pereira G, Ibrahim B
  • Molecular Systems Biology , 1/ 2012 , Volume 8 , pages: 582 , PubMed ID: 22580890
  • Molecular Biology of Centrosomes and Cilia, German Cancer Research Center, DKFZ-ZMBH Alliance, Heidelberg, Germany.
  • The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1-Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1-Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1-Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition.
Contributors
Submitter of the first revision: Bashar Ibrahim
Submitter of this revision: Rahuman Sheriff
Modellers: Rahuman Sheriff, Bashar Ibrahim

Metadata information

is (2 statements)
BioModels Database MODEL1202090002
BioModels Database BIOMD0000000699

isDescribedBy (2 statements)
hasTaxon (1 statement)
hasProperty (4 statements)

Curation status
Curated


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MODEL1202090002_Fig5 i.sedml SEDML file to reproduce Figure 5 i in the reference publication 6.78 KB Preview | Download
MODEL1202090002_Figure5_i.cps Copasi file for reproducing figure 5 I in the reference publication. 312.51 KB Preview | Download

  • Model originally submitted by : Bashar Ibrahim
  • Submitted: Feb 9, 2012 7:14:06 PM
  • Last Modified: May 21, 2018 3:49:22 PM
Revisions
  • Version: 3 public model Download this version
    • Submitted on: May 21, 2018 3:49:22 PM
    • Submitted by: Rahuman Sheriff
    • With comment: Notes updated using online editor.
  • Version: 2 public model Download this version
    • Submitted on: May 10, 2012 2:50:01 PM
    • Submitted by: Bashar Ibrahim
    • With comment: Current version of Caydasi2012_SPOC_UbiquitousAssociation
  • Version: 1 public model Download this version
    • Submitted on: Feb 9, 2012 7:14:06 PM
    • Submitted by: Bashar Ibrahim
    • With comment: Original import of MODEL1202090002.xml.origin

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Legends
: Variable used inside SBML models


Species
Reactions
Reactions Rate Parameters
Bfa1P5 + SPB_B => B_Bfa1P5 c3*(konB*SPB_B*Bfa1P5-koffB*B_Bfa1P5) konB = 1250000.0 l/(mol*s); koffB = 0.0012 1/s
Bfa1P5 + Tem1GDP => Bfa1P5_Tem1GDP c2*(alpha*konB5T*Bfa1P5*Tem1GDP-koffBT*Bfa1P5_Tem1GDP) alpha = 1.0 1; koffBT = 0.183 1/s; konB5T = 7000000.0 l/(mol*s)
Bfa1P4 + Tem1GDP => Bfa1P4_Tem1GDP c2*(alpha*konB4T*Bfa1P4*Tem1GDP-koffBT*Bfa1P4_Tem1GDP) konB4T = 3.65E7 l/(mol*s); alpha = 1.0 1; koffBT = 0.183 1/s
Bfa1P4 + SPB_B => B_Bfa1P4 c3*(konB4*SPB_B*Bfa1P4-koffB4*B_Bfa1P4) koffB4 = 0.0365 1/s; konB4 = 20000.0 l/(mol*s)
B_Bfa1_Tem1GTP => B_Bfa1_Tem1GDP c3*khydBT*B_Bfa1_Tem1GTP khydBT = 2.0 1/s
Bfa1 + Tem1GDP => Bfa1_Tem1GDP c2*(alpha*konBT*Bfa1*Tem1GDP-koffBT*Bfa1_Tem1GDP) alpha = 1.0 1; koffBT = 0.183 1/s; konBT = 3.65E7 l/(mol*s)
Bfa1_Tem1GDP => Bfa1P4_Tem1GDP c2*u*kfKin4Cyto*Bfa1_Tem1GDP kfKin4Cyto = 0.09 1/s; u = 1.0 1
Inactive_Tem1_in_the_cytosol = (Tem1GDP+Bfa1_Tem1GDP+Bfa1P4_Tem1GDP+Bfa1P5_Tem1GDP)*c2*avogadro [] avogadro = 6.0221415E23
Bfa1P4 => Bfa1 c2*krKin4*Bfa1P4 krKin4 = 0.0251 1/s
Curator's comment:
(added: 11 May 2018, 18:15:49, updated: 11 May 2018, 18:15:49)
Figure 5 of the referenced publication has been reproduced using Copasi 4.22.