S-BIAD229:IM1alpha
Lim et al/Figure 1/Figure 1ab/Figure 1a-MCs AT1.03/EoN/Figure 1a_01_EoN.lsm
Study Title: Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening
By:
Organism: Arabidopsis thaliana (thale cress)
License: CC0
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Image Dimensions : 512 x 512
Physical Dimensions : 179.2µm x 179.2µm
Pixel/Voxel Size : 0.35 µm x 0.35 µm x 0.0001 fm
Number of Channels : 5
Number of Time Points : 2
Characteristics [Organism] : Arabidopsis thaliana
Term Source 1 REF : Wild type mesophyll cell
Term Source 1 Accession : # leave blank
Characteristics [Cell Line] : TKTP-AT1.03
Assay Name : Figure 1
Experimental Condition [ # fill in] : 20-22 day old plant
Comment : MgATP2- biosensor
Image File : Figure 1a_01_EoN.lsm
Comment [Image File Type] : raw image
Channels : The ATP sensor AT1.03 confocal setting:Ex 515/Em 530(at 0.18 % of maximal laser power for all samples) (Channel 1: cp173Venus image); Ex 458/Em 500 (Channel 2: mseCFP image); Ex458/Em530(Channel 3: FRET image); Chlorophyll autofluorescence Ex 458/ Em 650 (Channel 4); TPMT (Channel 5); Merged (Channel 6)
SignificantBits : 12
BigEndian : true
Interleaved : false
ID : Pixels:0
Type : uint16
TimeIncrementUnit : s
TimeIncrement : 30.0
