P-GSE27298-2 - P-GSE27298-2
nucleic acid extraction protocol
Total RNA was extracted from 50~100 mg gills using Trizol (Invitrogen, Burlington, ON, Canada) isolation reagent following the manufacturer’s instructions. Total RNA concentration and quality were determined spectrophotometrically (Bioanalyzer2100, Angilent, British), with purity assessed (A260/A280 was ≥1.8; rRNA Ratio [28S/18S] values > 0.9; RNA integrity number RIN(Schroeder 2006) values > 8.0). Total RNA was treated with RQ1 RNase-free DNase (Promega). RNA samples were stored at -80˚C following isolation.