P-GSE21866-2 - P-GSE21866-2

growth protocol
Primary cell cultures were derived from tissue digestion with trypsin overnight at 4°C and the mixture was resuspended in a serum-free epithelial medium with high levels of amino acids (EMHA) and plated on dishes pre-coated with fibronectin and collagen.(Grafström R.C, Culture of Epithelial cells, 2nd ed, John Wiley and Sons Inc, NY, 2002). Cultures were transferred on regular tissue culture plastic at about 75 % confluence, and cells in passage 2 were used in the experiments.
Experiment E-GEOD-21866